RE:How to prevent bubbling in pressure cooker for immunohistochemistry
procedure.
Hi there Mark.
Really appreciate your feedback. I do use tween 20 in the retrieval solution
but actually thought it was to clean the slides, thus preventing background
staining. I did not know it prevents bubble
Re: How to prevent bubbling in pressure cooker for immunohistochemistry
procedure.
Maria
Thank you so much for your feedback. The instrument i use for the antigen
retrieval process is just household 6L Russek Hobbs pressure cooker. Before i
place my slides into this PC i do actually wait for it
Hi Subash
Imho your sections are not sufficiently adherent to the slide(s)
It is not M/W "bubbling" that causes your problem
If you use Superfrost Plus/lab-coated Silane or even double subbed slides (
thanks Ole!) AND dry your sections onto the slides efficiently, you will not
get section-loss.
N
