Re: [Histonet] Histonet Digest, Vol 194, Issue 7

2020-01-12 Thread LEROY H BROWN via Histonet
I am looking for a small sample of normal stomach for my IHC stain control. I am willing to trade any of my control blocks if you have a block you can spare. Thanks, LeRoy Brown HT(ASCP) HTL -Original Message- From: histonet-requ...@lists.utsouthwestern.edu Sent: Sunday, January 12,

[Histonet] X-gal staining

2020-01-12 Thread Amos Brooks via Histonet
> > > Hi, I'd like to concur with Carl Hobbs. With formalin fixed, paraffin embedded tissue, beta Galactosidase is definitely the way to go. It is just an antibody so you would do it like any other IHC. Amos Hi > No replies so far so.my pennyworth. > Imho ...no > beta Gal enzyme is

Re: [Histonet] X-gal staining

2020-01-12 Thread H. Etchevers via Histonet
I agree with Carl about the X-gal activity being destroyed by processing for paraffin sections (I'm a researcher, not a histologist). However, in our hands the anti-b-galactosidase antibody, reference A-11132, from ThermoFisher DOES work on paraffin sections (many others don't). It has been