[Histonet] Question about gelatin embedding

2020-01-17 Thread Alonso Martínez Canabal via Histonet
Hello, I am here again. I am wondering if someone has good experience embedding in gelatin-albumin for cryostat or vibratome sectioning. Specifically we use brain tissue and is common in free floating techniques non-attached parts of the same section float around and later that generates all

Re: [Histonet] Histonet Digest, Vol 194, Issue 12btattoo removal

2020-01-17 Thread Steve McClain via Histonet
I don not know any method for tattoo pigment removal. Aside from carbon black, most of the red, yellow, blue and green pigments are metal salts and polarize brightly. The inflammatory response may be vigorous, especially w repeat or Re-do of a tattoo. In some cases a pseudo-carcinoma or KA

[Histonet] Prepared slides of human skin with tattoos in xs

2020-01-17 Thread Jorge A. Santiago-Blay via Histonet
Hi: I teach college human anatomy and physiology and would like to have a slide or two of human skin with tattoos in cross section. Do you know where may I get a hold of those? All I use these days is an illustration from the web. Please, email me directly if you have constructive suggestion.

[Histonet] 3rd party block and slide storage fees

2020-01-17 Thread Olszewski, Dawn via Histonet
Hi everyone, We are a smaller hospital that stores our blocks, slides and files off site with a 3rd party storage facility. Does anyone have any information on fees for storage of blocks, slides, records and how much they charge to pick-up and deliver when needed and the names of any

[Histonet] FW: New Post: Strategies for Coping With the Shortage of Histotechs

2020-01-17 Thread Pam Barker via Histonet
Hi Histonetters! Here is my latest blog post on the NSH - Fixation on Histology site: "Strategies for Coping with the Shortage of Histotechs. TGIF. Have a wonderful weekend! Thanks-Pam From: Fixation on Histology [mailto:hi...@nsh.org] Sent: Friday, January 17, 2020 1:07 PM To:

Re: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis

2020-01-17 Thread Charles Riley via Histonet
The only way I know of is through lasers On Fri, Jan 17, 2020 at 12:25 PM John Garratt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > It is a pigment (ie carbon) and not like a soluble ink, so good luck with > that. > You could consult your histotech text book on exogenous pigment

Re: [Histonet] Skin Sample with Tattoo - need to remove for diagnosis

2020-01-17 Thread John Garratt via Histonet
It is a pigment (ie carbon) and not like a soluble ink, so good luck with that. You could consult your histotech text book on exogenous pigment removal and give the recipe a go. I will certainly be interested in other histonet replies. John www.cpqa.ca ‐‐‐ Original Message ‐‐‐ On

[Histonet] Skin Sample with Tattoo - need to remove for diagnosis

2020-01-17 Thread Donna Emge via Histonet
Hello fellow histonetters, We received a skin sample that has ink from a tattoo - the sample if from tattooed skin. One of our pathologists would like us to see if we can get rid of the tattoo ink from the sections before H staining. Does anyone out there know how to do this? Thank you, Donna

[Histonet] VERBAGE FOR CUTTING MULTIPLE SPECIMENS ON MICROTOME

2020-01-17 Thread Baldwin, Kathy via Histonet
Standard protocol for replacing microtome blades anybody have this or can help?? Thanks S Kathy Baldwin ASCP, SCT Memorial Hospital and Health Care Center Pathology and Cytology Manager Ph 812-996-0210 Fax 812-996-0232 CONFIDENTIALITY NOTICE: This e-mail message, including any attachments, is

[Histonet] WVU Medicine

2020-01-17 Thread Hall, Jessica via Histonet
Good Morning, We have recently opened a new Histotechnologist position with WVU Medicine! This position is eligible for a generous sign-on bonus, full comprehensive benefit package, and a competitive salary range. I invite you to contact me with any questions about this exciting

Re: [Histonet] Cell block preparations

2020-01-17 Thread Tony Reilly via Histonet
Some predictive text issues like lyse and nucleated. Sent from my iPhone > On 17 Jan 2020, at 8:24 am, Tony Henwood (SCHN) via Histonet > wrote: > > Hi Jennifer, > > I have had excellent success with lysing the red blood cells (using Isotonic > Ammonium Chloride) prior to cell block

Re: [Histonet] Cell block preparations

2020-01-17 Thread Tony Reilly via Histonet
Hi Charles A method my EM scientist used many years ago was quite simplistic and he thought it worked well. You simply centrifuge the specimen, add a set volume of deionised water, mix to lose the red cells, then add an equal volume of double strength normal saline to create a suspension in