Hi the list.
Do you know alternativ labels for zebra printer TLP 2742, connected to
dako agilent autostainer plus ? Did you try it ?
It should have the same size, and same distance between labels, and be
resistant to solvant and other chemicals, and thermal printed
(instead of Agilent dako
Gelatin embedding is easy. You infiltrate the specimen and then fix it again in
formaldehyde to cross-link the gelatin molecules and make the whole mass
isoluble in water. You can than cut frozen sections of any kind: cryostat, or
with an old-fashioned freezing microtome collecting thawed sectio
Good points but:
1. Good QC of the technique is required. The plasma has been tested for known
infectious agents and (in Australia) has been treated to remove/kill these
organisms. When we received the expired plasma, usually in a bag, we
immediately aliquot to smaller volumes and store frozen.
Tony has great point. I used to use Histogel (research for cell lines but
applicable to cell block). Switched to Low-melt agarose because of the temp
consideration on unfixed cells. Low-temp is a little misleading. Melts (in
oven at over 60 degrees) but COOL IT and remains liquid at 37degree
Good point regarding the molecular and another good reason to move away from
plasma. Plus taking plasma, even outdated plasma, is fraught with ethical and
regularity problems which are best to avoid now that ever milliliter of blood
product needs to be accounted for.
www.cpqa.ca
‐‐‐ Origi
Great helpful information.
We use plasma /thrombin.
It’s easy to use, and we have plenty of access to it from our blood bank.
However, we are looking to switch to Gel.
Why?
1. If you don’t keep an eye on your plasma, at some point you could see fungi
in your specimen with a chance you might t
Hi all,
Be careful of using cell block matrix that requires heat to solubilise the
matrix (eg agar or other commercial matrixes like Histogel).
Adding a heated matrix to unfixed, or even formalin fixed, material can
denature some antigens (eg CEA) resulting in a false negative IPX.
Unfortunatel
http://www.avantec.fr/content/dam/tfs/SDG/APD/APD%20Documents/Product%20Manuals%20&%20Specifications/Histology%20Equipment%20and%20Supplies/Embedding%20Cassettes%20and%20Molds/92957066-Richard-Allan-Scientific-HistoGel-Instructions-for-Use.pdf
The above link will help.
www.cpqa.ca
‐‐‐ Ori
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Anybody has validated procedure for histogel
Sent from my iPhone
> On Jan 23, 2020, at 1:07 PM, John Garratt via Histonet
> wrote:
>
> Hi Terri, I suggest you use Histogel for block preparation. It works
> exceptionally well, it is good for IHC and does not have the pitfalls of
> plasma/thr
Terri,
I agree with John on the use of Histogel. I use it routinely for all the
cell blocks and tiny matrix samples that come down and I get excellent
results.
I do a lot of IHC and the results are good.
Respectfully,
Colleen
On Thu, Jan 23, 2020 at 11:51 AM John Garratt via Histonet <
histo
Hi Terri, I suggest you use Histogel for block preparation. It works
exceptionally well, it is good for IHC and does not have the pitfalls of
plasma/thrombin.
Plasma/thrombin does work well for cell blocks but you will have to consider an
ethical and safe source for your plasma.
The instructions
Hi fellow Histonetters - I'm in need of some help, please
Background - We currently use agar to capture our scant cell blocks for
processing. I am unfamiliar with the Plasma/Thrombin method of cell block
preparation and am interested in comparing it to our current method
Request - Could you pleas
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