Re: [Histonet] FW: Tissue disposal

2020-01-29 Thread Thomas Podawiltz via Histonet
After decanting the formalin off our tissue is bagged in biohazard bags that are in a biohazard box. The box is labeled pathology waste for incineration only.  On Wednesday, January 29, 2020, 02:52:17 PM EST, Rathborne, Toni via Histonet wrote: Stericycle will also take your

Re: [Histonet] IHC on Pig Tissues

2020-01-29 Thread Liz Chlipala via Histonet
Lori You can't use a dual label detection system. We have found most mouse polymers will cross react with porcine tissue. We know that the dako one does. You need to use the rabbit envision for rabbit monoclonal antibodies or with mouse monoclonals you need to use a rabbit anti-mouse and

[Histonet] IHC on Pig Tissues

2020-01-29 Thread Lori Sorrells via Histonet
Good afternoon- Has anyone run into problems when trying to perform IHC using rabbit mono or polyclonal antibodies on pig tissues? I typically do not have any issues with nonspecific staining or high background when using mouse monoclonal antibodies. I have used a variety of blockers

Re: [Histonet] FW: Tissue disposal

2020-01-29 Thread Rathborne, Toni via Histonet
 Stericycle will also take your blocks and slides when you're ready to discard them. Toni NOTICE: This e-mail and its attachments, if any, may contain legally privileged and/or confidential information

[Histonet] Full-time Job Opening at Nationwide Children's Hospital

2020-01-29 Thread Pairan, Kelly via Histonet
Good Afternoon Histoland, We are looking for a full-time histotechnician or histotechnologist at Nationwide Children's Hospital. Our lab is open Monday to Friday from 3:30am until 6pm. We rotate on-call Saturdays and Holidays. The job is posted at :

[Histonet] Germinoma Control Blocks

2020-01-29 Thread Pairan, Kelly via Histonet
Good Afternoon, We are looking for a few germinoma tissue control blocks. If you happen to have some you can spare, please email me at kelly.pai...@nationwidechildrens.org. We may have something you need and would be happy to do a trade. Thanks,

Re: [Histonet] Tissue disposal

2020-01-29 Thread Dieringer, Connie J via Histonet
Eileen, I now work at a smaller facility, but when I worked for a large hospital, we did the same as what you described. We would strain off the formalin and then dispose of the tissue in large biohazard bags and a waste company would come and pick it up. Connie Dieringer, HTL(ASCP) Chief

[Histonet] Tissue disposal

2020-01-29 Thread Eileen Akemi Allison via Histonet
Good morning Histopeeps! After reviewing our Policy Procedure Manual, our manager is questioning the bags we are using for disposing our tissues after diagnosis is complete. We are separating the tissue from the formalin by straining the excess formalin and neutralizing it. We then put the