Dear Ed,
Adequate fixation is important. Formaldehyde penetrates quickly but reacts
slowly with proteins. 4% formaldehyde made by depolymerizing paraformaldehyde
(an insoluble high polymer) is the same as formaldehyde made by 10X dilution of
formalin (a mixture of soluble low polymers).
For
Hi Ed,
I have been using the 30% sucrose technique to cryoprotect animal tissue
for over 40 years without any problem. Did the tissue sink to the bottom of
the specimens jar? After sinking, I blot the excess sucrose from the tissue
on a paper towel before transfering to OCT. What is your procedure
Automation is a wonderful thing but it is only a replacement for what people
used to do by hand.
We have incubators that can be set to 60C and we have a Rube Goldberg-ized
microwave oven with
a thermal controller and relays (and the not-to-be-forgotten flyback diode) and
a K-type thermal probe
You need to add sucrose to your PFA we use 4%PFA+4%Sucrose to fix and then
cryoprotect in 30% sucrose which all need to be prepared in Phosphate buffer
solution NO saline if you message me directly I would be happy to share
our SOP this was a very hard thing to learn not a lot in
As a research lab, we sometimes would like to use paraformaldehyde-fixed but
non-paraffin embedded tissues; paraffin embedding alters antigens and
necessitates antigen retrieval, but simple fixation does not. We have done the
traditional 30% sucrose before OCT and freezing, with cryostat
Hi Garrey,
The answer is “it depends”. What you do when a processor fails depends on the
failure point. If the tissue is still in dehydrant it gets treated differently
than if it fails in the intermediate solvent.
Paula
Sent from my iPhone
> On Jul 4, 2020, at 10:08 AM, Garrey Faller
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Happy 4th to all.
Does anyone have a procedure on what to do when a tissue processor fails or
alarms. I want to learn more about the science behind tissue processing so I
know what to do when the machine fails. This happened to a friend recently and
I want to prevent my tissues/biopsies from
I was curious if there is a source, publication, report, study, general
information, regarding the use of lab assistants in the histology lab.
I would like the know the percentage of labs that utilize assistants versus
labs with techs only. I would also like to know that for labs that do
