Here are a couple of short videos that might be of interest. - John
https://www.bing.com/videos/search?q=amyloid+birefringence&&view=detail&mid=0663C1FAAFFA319FD8EA0663C1FAAFFA319FD8EA&&FORM=VRDGAR&ru=%2Fvideos%2Fsearch%3Fq%3Damyloid%2Bbirefringence%26FORM%3DHDRSC3
https://www.bing.com/videos/sea
I would add three additional pointers:
1) Assess in a darkened room with eyes acclimated to the dark;
2) Use a powerful light source--the apple green birefringence will be more
obvious and easier to discriminate from the white of collegen; and
3) Assessment sensitivity is further increased if inste
I neglected to mention sections should be cut at 10um for best results.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center
-Original Message-
From: Morken, Timothy via Histonet
Sent: Tuesday, July 28, 2020 1
Ken, Yes, polarized light and apple green birefringence is diagnostic for
amyloid with congo red and is the best practice. If you have a problem with
known control slides there are two possibilities: 1) make up fresh solution.
The pH has to be right. Or 2) try other control slides. Maybe you cu
Hi everyone. I was wondering if anyone out there has any experience with
diagnosing Amyloid tissue using Congo Red stained Kidney using polarized
lenses. Is it common to use polarized light to detect Amyloid deposits? Does
the absence of the "apple green birefringence" indicate a problem with
