[Histonet] Mart-1 rapid test for Mohs specimens

2020-08-13 Thread Anne Murvosh via Histonet
I'm working up a price comparison for NovoDiax's rapid 10 minute Mart- 1 stain for melanoma on Mohs specimens. The doctors would like me to price shop but I only know of 40 minute stains which is too long. Does anyone know of any other companies running a rapid 10-15 minute Mart- 1 stain?

[Histonet] Bubbles noticed on Bond-Max

2020-08-13 Thread Eddie Martin via Histonet
Hi Tim, From a user perspective: The slides you’re using may be a bad lot causing hydrophobic staining or you’re not cleaning your covertiles correctly. The wells on the assembly might also not be pulling adequately. Have you cleaned the assembly recently, particularly the wells? You could

Re: [Histonet] Blank Spots on IHC- Bubbles on Bond

2020-08-13 Thread Greg Dobbin via Histonet
Hi Tim, The usual source of bubbles is a deteriorating syringe or lines or valve. Have you observed the syringe while running the Clean Fluidics maintenance function? There can be some "micro" bubbles in the syringe on first draw of a reagent however, if working properly these tiny bubbles quickly

Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

2020-08-13 Thread Morken, Timothy via Histonet
Joanna, thanks! You are the first who said they had seen it before. I have a Leica tech coming in tomorrow to check out the syringes and lines. That was my thought too. Tim Morken Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of Pathology UC San Francisco Medical

Re: [Histonet] Blank spots on IHC tissues - bubbles on Bond

2020-08-13 Thread Bartczak, Joanna via Histonet
I have seen this phenomenon years ago on the Bond III when I worked at a different laboratory. If I remember correctly, Leica had to replace the syringes and lines as air bubbles were being introduced when aspirating reagents. I would definitely contact Leica technical service and let them