I'm working up a price comparison for NovoDiax's rapid 10 minute Mart- 1 stain
for melanoma on Mohs specimens. The doctors would like me to price shop but I
only know of 40 minute stains which is too long. Does anyone know of any other
companies running a rapid 10-15 minute Mart- 1 stain? Thanks
Hi Tim,
From a user perspective: The slides you’re using may be a bad lot causing
hydrophobic staining or you’re not cleaning your covertiles correctly. The
wells on the assembly might also not be pulling adequately. Have you cleaned
the assembly recently, particularly the wells? You could use
Hi Tim,
The usual source of bubbles is a deteriorating syringe or lines or valve.
Have you observed the syringe while running the Clean Fluidics maintenance
function? There can be some "micro" bubbles in the syringe on first draw of
a reagent however, if working properly these tiny bubbles quickly
Joanna, thanks! You are the first who said they had seen it before. I have a
Leica tech coming in tomorrow to check out the syringes and lines. That was my
thought too.
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Cente
I have seen this phenomenon years ago on the Bond III when I worked at a
different laboratory. If I remember correctly, Leica had to replace the
syringes and lines as air bubbles were being introduced when aspirating
reagents.
I would definitely contact Leica technical service and let them inve