[Histonet] Floaters

2020-12-07 Thread John Frazier via Histonet
Here is a reference link to floaters. My experience with tissue floaters is that most come from staining baths. However, some manufacturers of stainers claim there is significance with these floaters. My experience is that Pathologists read through the floaters and for the most part they don't

[Histonet] Crusting lesions and thick cattle skin

2020-12-07 Thread Rhonda McCormick via Histonet
Hi!I'm looking for recommendations for cutting FFPE blocks that contain thick cattle hide/skin and crusting lesions. What do you all use to soften this kind of tissue?  Our lab has used Millifex, fabric softener, and nair.  We read ammonia may work to rehydrate the tissue enough to get a decent

Re: [Histonet] Floaters

2020-12-07 Thread John Garratt via Histonet
Interesting thread. Thanks for kicking it off. Does anybody have a reference that cites diagnostic errors caused by floaters / contamination from other specimens? John On Mon, Dec 7, 2020 at 9:15 AM, Joe W. Walker, Jr. via Histonet wrote: > Don’t shoot the messenger. :) > > Joe W. Walker,

Re: [Histonet] Floaters

2020-12-07 Thread Joe W. Walker, Jr. via Histonet
Don’t shoot the messenger. :) Joe W. Walker, Jr. MS, SCT(ASCP) Anatomical Pathology and Interim Phlebotomy Manager Rutland Regional Medical Center 160 Allen Street, Rutland, VT 05701 P 802.747.1790  F 802.747.6525 joewal...@rrmc.org, www.rrmc.org -Original Message- From: Terri Braud via

Re: [Histonet] Floaters

2020-12-07 Thread Terri Braud via Histonet
60% of floaters from the water bath? I find that really hard to believe. The Gephardt and Zarbo CAP study from 1996 showed reported the results of a Q-Probes study of 275 laboratories and documented a frequency of contamination of between 0.6% and 2.9%, depending on the study method. Their