In my experience soaking the block in water works just as well as surface
decal. If you need to you can take the bone back, by reverse processing. Run
the block through the clean cycle back to water, then leave the bone in NBF for
24-48hrs. The fixative will protect the tissue when you put it ba
Use surface decalcification:
Surface the block, then remove from the microtome. Do not use the
microtome for another block, so as to avoid adjusting the position of
the block face to the knife.
Place the block face down in 4% nitric acid for 2 hours or longer.
Rinse the block and cool down
To Histopeeps,
Does anyone know if there is a method to decalcify bone once it is FFPE?
Thank you,
Pat L
George Washington University
Washington, DC
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