We have not encountered a covertile artifact with our Bond III, which we've had
for over 10 years. It may be that the covertiles need to be replaced or cleaned
more, but we have not experienced this. We also place a control tissue at the
top of the slide, and often have large patient sections
Hi,
The artifact you are seeing is a well-known artifact of the Leica covertile
design.
It does limit the available footprint for placing samples.
Hope this helps.
Regan
Regan Fulton, M.D., Ph.D.
CEO and Co-Founder
Array Science, LLC
475 Gate 5 Road, #100
Sausalito, CA 94965
(415) 577-7360
Paula,
Regardless of the position of the tissue: control/ patient ‘s tissue, most
likely is the distribution of the reagents during staining, isn’t adequate.
Syringe, staining reagents expiration, calibration, etc., Have you contacted
service from Leica? Hope the solution is found,
Thanks,
Hello everyone,
We have the Bond III and we ran an S100. We placed 2 serial sections
vertically on the slide with the control tissue at the top for staining. The
patient's section at the top underneath the control tissue had background
staining, while the section on the bottom of that had no
Hi Brian,
I regularly use epon for TEM work. Even though the instructions are good for
some climates , epon is particular.
Here in Houston, I let my blocks cure for 5 to 7 days depending on how humid or
rainy it is. When I press a fingernail into the block , if it leaves a mark I
know it
