[Histonet] salary ranges

2022-10-13 Thread Dorothy Glass via Histonet
What are the salary ranges for HT and HTL  as related to years of experience? Also I guess what region you live ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Reconstituting antibodies and dilutions

2022-10-13 Thread Gudrun Lang via Histonet
500 µg in 1000µl is the same as 100µg in 200µl So I would reconstitute the 100 µg with 200 µl of water to get the liquid antibody-concentrate. Then I would make the working-dilution: eg. 5µl of concentrate + 5000µl buffer The correct way would be 5 µl + 4995 µl, but in practice this does'nt mat

[Histonet] Reconstituting antibodies and dilutions

2022-10-13 Thread Charles Riley via Histonet
When calculating the dilution of an antibody when do start? i.e. If i have 100 ug of lyophilized antibody and its recommended reconstitution is 500 ug/ml How do I mix a 1:1000 dilution? I am thinking it's adding 2mL diH20 to create the 500 ug/mL and then diluting the sample. And I would be able