Hi Charles
I have done this stain many times as an abbreviated version of Movats
Pentachrome.
You are correct in thinking the van gieson will affect the AB stain due to the
acid.
As in the Movat stain do AB first then place slides in alcoholic ammonia for 1
hour to convert AB to insoluble
Just curious, why not do a Movat’s Pentachome? It gives you everything you are
looking for and more.
Best regards,
Akemi Allison-Tacha, BS, Ht, HTL
akemiat3...@gmail.com
Sent from my iPhone
> On Mar 9, 2023, at 11:19 AM, Paula Keene Pierce via Histonet
> wrote:
>
> Hi,
> if they are
Hi,
if they are wanting to stain elastic, Alcian blue, and Van Gieson, I recommend
doing the Verhoff elastic stain first, then the Alcian blue, and then the Van
Gieson.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N
Blue Lake DriveNorman, OK 73069PH
I am working with a researcher and they saw an article where someone
performed an Alcian Blue and elastic van Gieson double stain. If anyone
has done this before can you provide your protocol. I am trying to figure
out the best way to perform this process to get the best staining results.
Here
I remember adding a couple drops of ETOH to the water bath; it's been a while
so I am not sure the exact protocol. (it will spin the tissue).
Virginia Kurth, HT, (ASCP)CM
UW Health Surgical Pathology
Madison, WI 53792
-Original Message-
From: Renee Fisher via Histonet
Sent:
Hi Histotechs,
Please join us at Sea Palms Resort on St Simons Island, GA for our 50th
anniversary meeting. The links to the program and registration are below.
Rooms will go fast so make your reservations soon. The hotel rates and phone
number to call are on our website at
Hi Renee,
Brains are notorious for being difficult. Processing has to be just so,
embedding has to be just so, and sectioning? Well, just plain tricky.
It sounds like your water bath may be a smidge too cool. Brains are picky that
way. Water bath too warm? Poof! The section explodes. Too
We are having issues with mice and rat brain sections. There are folds in the
tissue. We just can’t seem to get a flat section without folds. Any suggestions?
Sent from my iPhone
___
Histonet mailing list
Histonet@lists.utsouthwestern.edu
