Hello gang
Jores has several references found in a Google search, including a post right
here on the Histonet from 2002.
It contains Chloral Hydrate which is very difficult to find.
Do a search and have a look. Several suggestions.
AnnieinArabia (now retired in Africa)
Sent from my iPhone
Hi,
The thicker the section the more likely it will be to fall off. Frozen
sections already love to fall off slides. You should cut them a lot
thinner. 4 to 10 um should be the thickness, especially for frozens.
If you want very thick sections as you describe, you would be better
off
Hi,
I think the answer is as so often: it depends. Detergens solves membranes
partly and leads to a higher permeabilization of the tissue. Some antigens may
take advantage of that, some may not need it.
Higher permeability is good for detection with high molecular complexes.
Gudrun Lang
