I'd like to preface that I haven't done any Alexaflour testing in just over 10
years.
The simplest response may prefer using a conjugated secondary if you're
planning to do multiplex or multicolor analysis.
If I was limited in commercially available options for primaries needed for the
test
Hi,
Direct IiF (a fluorescent conjugated primary) is certainly easier, but
there are some reasons one might prefer using an indirect method (using a
conjugated secondary). Indirect methods allow the use of a different
wavelength to be used simply by switching the secondary. It is also
Naira
Did you get feedback on the tissue processing protocols for small and large
animal tissues? If so I would be interested in obtaining those from you?
Thanks in advance if you can forward them on to me.
Kate
-Original Message-
From: histonet-requ...@lists.utsouthwestern.edu
If you want a stronger signal I would probably use the secondary antibody vs
using a directly conjugated CD11c.
You could use a directly conjugated one as you would for flow cytometry but it
won't give as much amplification as it would with a secondary step built in.
Katy
-Original
Why would one decide to use a primary antibody along with a secondary
antibody rather than a primary antibody conjugated with the secondary?
Example. I have a researcher who wants to do CD11C staining with
Alexafluor488
Is it better to buy and use a primary antibody CD11C conjugated with
