Re: [Histonet] [EXT] Reagent alcohols and tissue processors

2024-03-28 Thread Colleen Forster via Histonet
Paula, I start my processor at 80%. I am in research and often the samples have been fixed and are put into 70-80% ethanol for holding or transport. Starting with 80% steps right in line with the samples. I rarely see a schedule that starts at 50%. However, I do have a couple labs that ask me for

[Histonet] ThrowBack Thursday - Throwback to the last time you updated your resume! Day 4 of RELIA Resume Tune-UP Week!

2024-03-28 Thread relia1--- via Histonet
Hi Histopeeps! Happy Thursday! Let's Throwback Thursday to the last time you updated your resume! Yep It's Day 4 of RELIA Resume Tune-up WEEK! I have already tuned up some resumes for some amazing histopeeps and am ready for more! REMEMBER! There are lots of reasons to have an updated resume!

Re: [Histonet] [EXT] Reagent alcohols and tissue processors

2024-03-28 Thread Paula Sicurello via Histonet
Hi Kara, I do like the VIPs.   Besides getting rid of the NBF, the alcohols are getting rid of the water as well. I learned EM first, so my processing thoughts are probably biased by that. Paula  Yahoo Mail: Search, Organize, Conquer On Thu, Mar 28, 2024 at 6:14 AM, Kara, Phillip wrote: S

Re: [Histonet] [EXT] Reagent alcohols and tissue processors

2024-03-28 Thread Kara, Phillip via Histonet
So for your first one I love the Sakura Tissue-Tek VIP. Easy to use, easy maintenance, and very customizable for programed runs. Every lab I have been in has always started at 70. It saves the techs extra time mixing up dilutions because you can always find RTU 70%. It also helps I have never ha

[Histonet] Reagent alcohols and tissue processors

2024-03-28 Thread Paula Sicurello via Histonet
Good Morning, My question two part: Best tissue processor ever and why? What is percentage is your first alcohol step? I see that lots of places start at 70%  but I favor a lower %.  One because it's gentler on the tissue, and two because 10% NBF precipitates out if you start at 70%. Thanks for y