We wick our sections dry and then incubate for 15 to 20 minutes @ 70-75 degrees
in a convection oven. We tried lower temps and longer baking times for many of
our markers, but we didn't see any discernable staining difference.
Thank you,
Brian Cooper, HT, QIHC (ASCP)
Histology/EM Supervisor
Ch
Interesting. I never baked my slides. Left air dry o/n on room. Next day
started with deparaffinization steps 2x30min. My most AR where in the
citrate buffer in the water bath with 92C(if I remember correctly) for 37
minutes then 12 minutes cooling at room temperature. Never had a problem.
Naira
O
Good morning Histo Peeps!
I am taking a poll on what various labs are using for temperature and times for
IHC’s slides which are baked in the oven. It has been my experience while
working at Biocare Medical, we would 1st wick excess water off the slides, then
tap the rack of slides on paper; t
Hello,
Another part of the process is to wick the tissue and place in front of a fan
for about 10mins before placing in the 60 degrees oven. Just a suggestion.
Robyn
-Original Message-
From: Catherine Anne Heyward via Histonet
Sent: Thursday, August 8, 2024 5:10 AM
To: histonet@lists.ut
Hi,
If HIER at 90 degrees C is causing tissue lifting, you could try a lower
temperature for longer. I was recommended 60 degrees overnight for
bone/cartilage and have had far fewer problems with this gentler treatment.
Perhaps this would suit the skin samples too.
Best wishes,
Cath
Catheri
