[Histonet] Microsporidia

Dear Friends, Can anyone tell me how to demonstrate Micrsporidia in Deudenal biopsy.For me the modified Brown and bren gram is not working.if u have any other method pls let me know. with regrads, Aazath

[Histonet] PAP

Dear friends, does anyone have rapid PAP(cytostain) preparation,for rapid PAP staining. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals chennai India

[Histonet] RE: Histonet Digest, Vol 80, Issue 31

Dear Friends, Does anyone have rehydration technique of air dried smear which can be stained for pap. Aazathraj.P Technical Officer Department of Histopathology and Cytology Apollo Hospitals -chennai India

[Histonet] Artifacts in histology section

Dear Friends, I am an Histology Technologist. I am having a problem here,while sectioning am not seeing and scoring artifacts on the section but in the microscope am seeing a tearing artifacts particularly in endoscopy biopsies. am not able to locate where is the problem,is

[Histonet] problem in IF stains

Dear Friends, I am having a problem in IF stains on Renal Bx.In direct immunofloresence on the frozen sections does not have disered contrast and many of the cases its falsely negative. Can you people tell me the possible mistake which may cause these kind of effects on the IF

[Histonet] Fading of Histology slides

Dear Colleagues , I am an Histopathology Technologist .We use DPX (merck) as the mounting medium for the the light microscopy slides. Particularly HE slides gets faded very fast with min 3 -4 month. Is there any other alternative other than gum dammar which we are not

[Histonet] Processing Of CSF

Dear Friends, Iam a Technical Officer in Apollo Hospitals India in Department of Histopathology and Cytology. I am having a problem in CSF processing for cytology. We are using Thermo Shandon 4- Cytocentrifuge for processing CSF and Urine. The yield is very less. We spin it with

[Histonet] HE preparation

Dear All, We are preparing harris heamtoxylin for HE in our laboratory. some time we get intense nuclear staining some time light weak.Does anybody know how to control a consistency in the strength . Aazath Technical Officer Apollo Hospitals India

[Histonet] (no subject)

Dear All, we have got a new leica multistainer ST 5020.we are using it for both HE and as well as PAP.Out of 34 station i have only 15 station for HE.Is anybody having a Staining protocol for HE which fits in 15 station with 2 water wash bath. Aazath Technical Officer Apollo