[Histonet] Horse Tendon Microtomy

2017-12-14 Thread Alan via Histonet
Dear All Thank you to everyone who replied to my recent request for help with the above. As these were particularly tough tendons we finally used a combination of KOH and a disposable tungsten blade which gave us fairly good sections, more suitable for scanning than our previous efforts. Thanks

[Histonet] Microtomy of Equine Tendon Blocks

2017-12-05 Thread Alan via Histonet
Greetings to all on Histonet. I have a particularly challenging question regarding the sectioning of Equine tendon material. My colleague and I recently received a number of TS tendon slices. Unfortunately they had been placed in 90% IDA for fixation approximately 10 years ago, where they remai

Re: [Histonet] Wright's stain

2016-12-26 Thread taylor alan via Histonet
> On 26 December 2016 at 22:15 taylor alan wrote: > > > Hi Lauren > > With reference to your enquiry for the preparation of Wrights stain from > Wrights stain powder > > I have found the original method of preparing Wrights stain in one of my > favourite 'historical' pathology tex

Re: [Histonet] Wright's stain

2016-12-26 Thread taylor alan via Histonet
Hi Lauren With reference to your enquiry for the preparation of Wrights stain from Wrights stain powder I have found the original method of preparing Wrights stain in one of my favourite 'historical' pathology text books. Practical Bacteriology, Bloodwork and Parasitology. Eighth Edition. Edited

Re: [Histonet] Reticulin Stain

2016-07-21 Thread taylor alan via Histonet
Hi All Here in our lab we routinely hand stain reticulin sections. Our preferred choice is the Gordon and Sweet method. The technique is easy to perform, diffing out can be easily controlled via the staining microscope and a variety of counterstains can be used in addition to the usual Neutral Red