Does anyone knows how to remove DAPI? I have some old sections with very
deteriorated DAPI. I would like to remove it and stain again, do someone
knows?
Thank you!
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And yes, I would like to remove the dapi residues without affecting the
overall Alexa staining.
Thank you!
-Mensaje original-
De: Alonso Martinez-Canabal [mailto:alonso.martinezcana...@utoronto.ca]
Enviado el: September-10-12 5:32 PM
Para: 'histonet@lists.utsouthwestern.edu'
Asunto
Hi
my boss requires me to counterstain some DAB immunohistochemistry with
methyl green. I use for that purpose the methyl green in aquos solution
0.5% in 4.2 acetate buffer. The problem is that after the washes I dehydrate
and the methyl green becomes extremely faint with the alcohols. My
I would like to start to use some form of pen to permanent label my
slides, the pencil marks somehow not very resistant to the immersion oil and
that have created some problems. EM and other brands have some commercially
available pens with solvent resistant ink. However, I am quite
