Re: [Histonet] cleaning old charged slides

Hello, Has anyone had success cleaning old charged slides which have become foggy over time. Thanks! ~Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Leitz Rotary Microtome

Hello, Does anyone have experience with a Leitz Minot Rotary Microtome No. 1212? I'm particularly interested in a source for troubleshooting problem sectioning. Thanking you in advance for your assistance. Best Regards, ~Atoska ___ Histonet mailing list

Re: [Histonet] Histonet Digest, Vol 188, Issue 17

Hello Sandra, Following are catalog #'s for Fisher substitutes that I received from their site: 12-545GP (50x35mm); 12-548-5RP (45x50mm). Ted Pella, Inc. Gold Seal 3327 www.expressmedicalsupply.com Erie-Scientific ER 35x50-1.5 & ER 45x50-1 Also try Brain Research Laboratories 4860-1

[Histonet] mouse pituitary

Hello, does anyone have a tried & proven method and/or pointers for mounting mouse pituitary for cryo sectioning which guarantees great cross sections? Thanks! ~Atoska Sent from Mail for Windows 10

Re: [Histonet] blades

Hello does anyone have a source for Personna Single-edge stainless steel razor blades? Our former vendor no longer stocks them. Thanks, Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] specimen storage cabinets

Hello, I work for a research facility and we currently have an archive of several formalin fixed samples stored in 500ml glass specimen jars; one of our PI's recently asked me to conduct a search for storage cabinets specifically designed to accommodate these samples. Is anyone aware of the

Re: [Histonet] Automated Slide Stainers

Hello, one of my department's researchers is in need of an automated slide stainer which accommodates 3"x2" microscope slides. If you have experience with such will you please contact me at your earliest convenience? Regards, Atos

Re: [Histonet] microscope slides

Good afternoon, does cleaning dust from adhesion superfrost plus microscope slides interfere with paraffin section adhesion? If not will someone be so kind as to share their tried and proven method ASAP? Thanks in advance. ~ Atoska ___ Histonet

[Histonet] RE: Hemo-De

Hello is anyone using Hemo=De as the clearing agent in the following stains: Bielschowsky's Method for Axis Cylinders Dendrites and Sevier-Munger Method for Neural Tissues? Regards. Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Hemo-De

Hello, has anyone use or are you currently using Hemo-De as the clearing agent in these stains: 1) Bielschowsky's Method for Axis Cylinders and Dendrites and/or 2) Sevier-Munger Method for Neural Tissues? Your prompt reply will be much appreciated. Regards. ~ Atoska

[Histonet] RE: Adipose Cryosections Fixation

hello! Will you either provide me with info protocol for fixation of adipose cryosections prior to staining and/or contact info for Dr. M. Rocio Sierra-Honigmann ASAP? Thank you kindly, Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] RE: Feline adipose tissue IHC

hello, I'm assisting a graduate student who's interested in info on source recommendations for the following macrophage markers which guarantee results on feline tissue: 1) AM3-K, 2) alpha1-antitrypsin and 3) Lysozyme. Your prompt replies with pertinent info will be greatly appreciated. ~ Atoska

[Histonet] RE: Microm HM 505 E Cryostat

hello, has anyone with this particular cryostat experienced frost build up on the top interior area located above the microtome? I've also noticed the temperature has for the past couple of weeks been reading ~ 3 - 4 C colder than temperature setting. Thus far it seems to still be sectioning just

[Histonet] Re: replacement Turtox Internal Ear, cochlea slides

Hello! please see below inquiry from a colleague. Thanks! Atoska Our histology class is looking for replacements for old Turtox (Guinea Pig) Internal Ear, cochlea slides and was wondering if anyone knew a company who supplied these slides or tissue blocks? I don't think it has to be guinea pig

[Histonet] RE: Lynn Burton, Galesburg, IL

Hello Lynn Burton of Animal Disease Lab, Galesburg, IL will you please contact me ASAP regarding a 2006 Histonet posting. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Re: chick embryo frozen sections

hello, will some one please give me pointers on successful methods used for cryosectioning bursa esophagus from approx. 21 day chick embryos (such as recommended temp. micron thickness, for yielding the best sections)? Thanks! Atoska ___ Histonet

[Histonet] Re: Acetone Fixation

hello, if any of you have acetone fixation incorporated into you frozen section H E staining protocol will you please advise me on adjustments necessary for routine staining protocol. Also, out of curiosity please enlighten me on the purpose for post sectioning acetone fixation on tissue samples

[Histonet] Re: tissue processor

hello, I'm in search of the best tissue processor on the market thats' most comparable to the Autotechnicon Ultra II. I specifically need it to be equipped with vacuum heat controlled processing and for it to possess the capability of processing 2.75 and/or 1.5 stainless steel round tissue

[Histonet] Re: Citadel 2000 Tissue Processor

Hello, will everyone who's used/using the Citadel 2000 Tissue Processor please share with me your opinions, pros/cons and etc ASAP? Thanks! Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re: Cynthia Favara

hello will someone please provide me with contact info for Cynthia Favara ASAP? I need to consult with her on a histonet archive post. Thanks! Atoska ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Re: heartworm processing protocol

hello, will someone with a tried proven protocol for processing heartworms for paraffin sectioning please share your protocol with me ASAP? My histonet archive search was unsuccessful. Thanks! Atoska ___ Histonet mailing list

[Histonet] RE: Tissue Capture Pen

hello, if any of you are using and/or have used the Ted Pella, Inc. Tissue Capture Pen will you be so kind as to share with me your experience (s), pros, cons and whatever else you deem necessary? Also, does it's use require special glass slides? Any info you can provide on it's use ASAP will

[Histonet] Re: tissue processors

hello, we are in the market for a new/replacement tissue processor. If you have experience and/or pertinent detailed info on any of the following will you please share with me ASAP? *1.*ThermoShandon Citadel 2000 Tissue Processor, *2.* Leica TP 1020 Automatic Tissue Processor, and last but