Re: [Histonet] Processing artifact - delayed start

We have had 2 reasons why we saw processing issues like this: 1. I recently found out on our VIP 5 they did not turn the level sensors on during install. These sensors are known to error so often, Sakura tells technicians to set the default to off. All the processor can sense is pressure and ti

Re: [Histonet] Retic help

Hi Steven, We ran into this issue and it was the pH of the water. We were using our sterile line from the facility but the pH was too high. We used tap and it worked fine. This will also effect your stock solutions if you make them yourself so be aware of that as well. Sent from my iPhone >

Re: [Histonet] Formalin pH

We pull the COA from the vendors website. Some are better than others but the are usually listed by lot number. I keep them in a network shared folder so they can be pulled by anyone that is asked for the evidence during inspection. Sent from my iPhone > On Sep 13, 2023, at 8:22 AM, "e...@pigs

Re: [Histonet] Tissue disoposal/formalin

We use SteriCycle. We decant off all formalin and put the tissue in double red bagged biohazard boxes for incineration. The boxes are hauled by them and we have manifests in triplicate that are retained by our Safety department. Anyone signing the manifest needs to attend the training for the Ma

[Histonet] New HRBP

This message is to announce that Drake Dargon, HR Business Partner, will be leaving Baystate Health effective May 16, 2023. for another opportunity. Drake joined the HRBP team in January 2022, providing support to the Pharmacy, Radiology and Laboratory teams. Chanel Duchaine, HRBP, will be taki

Re: [Histonet] Alarm system

ViewPoint - Mesa Labs Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786  Fax: 413-322-4790 charles.ba...@baystatehealth.org -Original Message- From: Katie Riley Sent: Thursday, March 2, 2023 1:54 PM To: his

Re: [Histonet] destain for IHC

Hi Nancy, We use our Acid alcohol solution from our AFB Stain. It pulls off the Eosin and leaves a faded Hematoxylin behind. We counterstain in Hematoxylin anyway so we find this protocol effective. Then we remove the deparaffinization step from our IHC protocol and run the slide. 1% Acid Alco

[Histonet] Automated ihc staining of bone

When we have section lifting during IHC, we will use a concentrated Poly-L Lysine solution. You can dilute this solution but instead we will use a direct application (wet the tip of a gloved finger with the solution, and wipe down the slide, let dry) on one of our charged slides. This may cause

Re: [Histonet] best paraffin

Over all of my testing I found there are several factors to consider when choosing paraffin: 1. Processor type 2. Tissue type 3. Use - embedding vs. processing The processor type my dictate limitations on paraffin selection. For example we were told not to use Paraplast Plus in a processor tha

[Histonet] IHC staining of cartilage

We use P8920 Sigma-Aldrich Poly-L-lysine solution. Great retention without excess background staining. You can prep a rack of slides in diluted solution but we put a gloved finger over the end of the bottle and and swipe it down the slide at full strength. Let the slide dry and move forward as y

Re: [Histonet] Multiple H&E stainer maintenance

Hi Leslie, I have attached our current log. You can augment it based off the solutions you are using and how many shifts you have. We have two stainers and each has their own log. Our stainers are connected to running water thus the drain lines that need cleaning. If you have any questions reac

Re: [Histonet] Sectioning problems with Fisher Replacement Paraffin

Hi Eric, We use a great product from Polyscientific, called "Infiltrating/Embedding Paraffin Prills" Item Number: c827 https://www.polyrnd.com/product/infiltratingembedding-paraffin-prills/. I am in no way affiliated with this company so this is just a simple Histotech review. As the name sugg

Re: [Histonet] Microtome Recommendations

Hi Kelly, We have tried several manufactures, as well as model types over the years. We have had sliding microtomes (Microm, no longer available), and rotary microtomes from, Leizt (old), AO (very old), and Leica. We have had several models from Leica and prefer their durability. If you are man

[Histonet] Paraffin Preference

Hi Paula, I have worked in a couple different labs and in one lab we used Paraplast Plus. Because of the DMSO, tissue infiltration is superb with that paraffin. Unfortunately DMSO is not good for stainless steel so when we had to replace our processor (now with a stainless retort) we also had t

Re: [Histonet] Toenail staining issues

We use poly-L-lysine. Works for us. P4707 Sigma-Aldrich Poly-L-lysine solution 0.01%, sterile-filtered, BioReagent, suitable for cell cultur Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786  Fax: 413-322-4790 charle

[Histonet] Ventana Benchmark Ultra

Hello Warda, As you know the Dako Autostainer and PT link set up works well but has a good amount of tech time involved in its use. We currently run the Dako as well as the Benchmark Ultra. The Ultra is a major upgrade in ease of use. The antigen retrieval is onboard with no tech time to switch

[Histonet] ISH policies

Hi Jen, What platform are you using? We use Ventana and have SOPs in place. If you have the Ultra Benchmarks I would be happy to help! Chuck Bacon, HTL(ASCP)CM Supervisor Histology Baystate Medical Center 361 Whitney Ave., Holyoke, MA 01040 Telephone: 413-322-4786  Fax: 413-322-4790 charles.ba

Re: [Histonet] Tissue processor advice

Hi Colleen, We have all VIPs here at BMC but I have used the Leica in the past at a previous position. We currently have 3 - E300s, 3 - VIP 5s, and 2 - VIP 6s. The VIPs are great and really only need preventive stuff like O rings and lubrication. A service company for us Avantik takes great car