Hello
Soak slides in xylene or equivalent solvent for sometimes till the
coverslip is loose, carefully remove the coverslip; you can remount the
slides with Permount.
You can dissolve the Permount in organic solvents, but the glue will be
dissolved and you have to remount the slide.
Bader
On
Hello
Methanol is added to reagent alcohol (ethyl alcohol) so that people do not
use for drinking purpose and for tax purposes. Alcohol (ethyl alcohol) has
federal tax just like liquor.
One can buy pure alcohol, but you have keep inventory and make sure it is
not misused.
Bader
On Tue, Jul 17,
LinkedIn
Bader Siddiki requested to add you as a connection on LinkedIn:
--
David,
I'd like to add you to my professional network on LinkedIn.
- Bader
Accept invitation from Bader Siddiki
http://www.linkedin.com/e/yvpgd1-gxm7sa8y
Hello Histonetters
We are in need of someone who can test two antibodies: CD71 and CD74 for us.
--IHC Frozen Tissue (Methanol fixed tonsil) for both antibodies.
--ImmunoFluorescence - Frozen tissue (Methanol fixed) We have FITC
conjugated antibodies.
Please contact us at : bade...@gmail.com
If you
Hello Histonetters
I have been reading about xylene, how dangerous it is.
Have you thought about using mounting medium made from Limonene.
Here is some info:
*AR-6504 Organo (Limonene) mount™:** *This mounting medium is made with
limonene a natural product from orange peels. It is good for
Hello Histonetters
I need your professional advice.
We are a small IHC and IF company looking for a good microscope.
We have investigated in Nikon;s they have 50i and 55i microscope that one
can use for IHC and IF with proper attachment, but pretty steep price.
Do you have any recommendation for a
Hello Histonetters
I hope you guys and gals are getting ready for the weekend, have a good one.
Few months ago we received an e-mail on histonet that there was a place on
internet one can purchase electronics at reasonable prices.
Did any order anything from them, are they for real.
I lost
Hello Laurie
Pi gives red color in IF, it stains DNA and RNA
PI excites at 535nmand emits at 615nm, producing a *red* fluorescence.
Therefore if you are using any chromophore at these wave lengths which emits
red color you will have problems.
By the way we also have several mounting mediums for
Hello
I have the same experience.
When I worked for a company and right after 9/11, we shipped antibodies to
Israel via Post office at ambient temp.
This package came back after 6-8 weeks or may be longer.
We do not know how they were stored at post office.
We tested the antibodies and could not
Everybody is writing about the plants in the histology lab.
May be I will share my experience with you in Biochemistry lab at MSU .
Many many years ago when I was a post doc in Biochemistry dept. I had lots
of plants in the window.
One day when I came from lunch, I saw unusual plants along with
Hello Histonets
I need some help and advice from you experts.
We are new in cutting formalin fixed paraffin embedded (FFPE) tissue
sections.
We need your advice.
We have a used Leitz 1512 microtome.
--- perhaps it needs service, can you recommend a good and reasonable
place.
what will
Need help, looking for knife/blade holder for Leitz 1512 Microtome.
Does anybody know where to buy this, thanks
Bader
e-mail address: bade...@gmail.com
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Hello
My experience is that most Immunofluorescence dyes do not withstand alcohol
and organic solvents dehydration.
You can use aqueous mounting medium with anti-fading agent.
Frozen brain tissue is tough to work with; since it contains tons of
lipids.
Lots of mounting mediums will produce air
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