RE: [Histonet] RE: Plain Vanilla Autostainer?

to do its thing! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

RE: [Histonet] hi-profile blades

I've been using Accu-edge blades from Sakura and have found nothing to equal them. We use low profile here, but high are the same quality. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723

[Histonet] Block release

are in Australia, Ireland, Canada, Puerto Rico or Peru please also answer (though I sort of know already) as we do get blocks from you also. Thanks Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL

RE: [Histonet] RE: New Cap Guidelines for Her2 and ER/PR

in Seattle (it's at home, not here at work) I can look it up or if someone has it handy, please chime in for those interested. Unofortunately, this was an underpopulated workshop, but very informative. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL

RE: [Histonet] Re: Masson's trichrom - problem with nuclei staining

We use Weigert's for 7 minutes and never more than a week either. As a precaution we keep it in the dark in a non-transparent container. That's the way I was always taught (26 years and counting) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710

RE: [Histonet] Warthin-Starry Stain

We use the modified Steiner - the kit from Sigma,to be precise. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

Re: [Histonet] Reagent Grade Alcohol Brands

!! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet -- Bernice Frederick HTL (ASCP) Pathology Core Facility Robert H Lurie Cancer Center Northwestern University Olson Room 8421 Chicago Il 60611 312-503-3723

RE: [Histonet] vanGieson-Trichrome staining protocol

And it looks so pretty (VVG/TRI)- especially on lung. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] PPE

We require gloves for sterile sectioning as we are usually taking sections for DNA and RNA (and a mask).Otherwise gloves etc are optional. Nothing for embedding. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL

RE: [Histonet] MAILING BIOPSIES

and extracted DNA ,so we have to know what to do with it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] RCC

RCC from Novacastra (Leica) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

RE: [Histonet] 72644.18148...@web111105.mail.gq1.yahoo.com

How about all of them? Being as it is April 1. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet

[Histonet] DAKO Her2 antibody

fixation etc. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

RE: [Histonet] Histology Stories

the blocks and or slides for these trials here at NU from the US as well as other countries. Makes for a varied and interesting job. And yes, I do remember the days of regular knives and making up my own Schiff's! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility

RE: [Histonet] TGF beta on FFPE

Which receptor? We run BR1,BR2and BR3 on FFPE as well as B1,B2 B3. Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] HE stainer/coverslippers

Vicki, We just ordered the Leica combo.We have the separate units of the Autostainer XL and CV5030. They now sell as a combo (a transfer station moves the racks) As we've relied on Leica for years, I expect we will be happy with the new unit. Bernice Bernice Frederick HTL (ASCP) Northwestern

RE: [Histonet] Re: Fite control slides

NSH may have some in their control bank. Newcomer Supply sells control slides. Sigma may also. BErnice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From

RE: [Histonet] Basement membranes

AKA Jones Method. Sigma sells a kit or you can make it all up yourself. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] (no subject)

Try Jackson Immunoreasearch. We've gotten a lot from them for our double stains. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] Ultra-Low Temp. Upright Freezer

on it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] COX-2

Richard, We use the COX2 for Cayman chemicals and have never had any issues with it. It stores at -20 once you rehydrate and aliquot. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723

RE: [Histonet] hood for manual special stains

but on zoonoses (the nasty ones) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

RE: [Histonet] Microtome Alignment

Microm sells a device called a histocollimeter, however, it only attaches to their microtomes to my knowledge. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message

RE: [Histonet] Fontana Masson

Rowley biochemical may also have it. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

RE: [Histonet] Whole human feet

Virtual autopsy web site? They have the whole body ct scanned and sliced. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] Embedding cell pellets

Try histogel from Richard Allan- available from Fisher.Easier than making up agarose! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] low profile blades and cutting angle

It's all I ever use and I've tried a bunch. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Calcium salts

Sodium thiosulfate removes excess silver and has been used in the Von Kossa technique for years as well as most,if not all silver stains we do. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312

[Histonet] TMA tape sectioning system

Does anyone out there remember who had the nifty sectioning tape for TMA's? Or does anyone use it? The vendor was at the NSH meeting last fall. Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421

RE: [Histonet] RE: OT: Books

So what does an Anne Preece go for? Can I retire too? I've got one of those Sheehan's too. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet

RE: [Histonet] RE: Sandersons rapid bone stain photo

Maybe the NSH hard tissue committee has a picture. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] problem eyes

We use it for fatty samples (we do lots of breast) and yes, it does work well. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] Tube station

I stayed near Russell Square station but liked Victoria despite the hustle and bustle ... Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun

RE: [Histonet] Tube station

Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Della

RE: [Histonet] RE: testing cutting ability--This is starting toReallyAnnoy me.

cleaning time etc means maybe 30 blocks in a day (we cut a fair amount of slides for correlative studies) Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From

RE: [Histonet] Training first time cutters

It varies in my opinion. Some people take longer to catch on. I think ability goes along with workload- I cut quickly and accurately whereas others cut slower and just as accurately. I find I can vary as to tissue also- it depends on the tech. Bernice Bernice Frederick HTL (ASCP) Northwestern

RE: [Histonet] uncertified techs in Histology

are classified at NU as Sr. Research techs, we have to really fight the U for salary and we are only in the middle of the scale, but we are in a very relaxed atmosphere. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421

[Histonet] Triple stain problem- antigen retreival conflict

-mouse) MAC-3: Ph9 (rat anti- mouse) Insulin: none ( guinea pig anti -swine) Any help will be appreciated!! Thanks, Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723

RE: [Histonet] uncertified techs in Histology

So take the registry exam Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun

RE: [Histonet] Re: Paraffin Blocks

the tissue for that trial. We have translational studies as far back as 1990 that I ma afraid the rest of the case is gone. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original

RE: [Histonet] Sectioning on different microtomes

I'm on the same page as you Jenaine- we get blocks from all over so we are constantly rearranging the block holder. We also do have a histocollimeter to get the Microms back in line (it doesn't fit on the Leicas. Berncie Bernice Frederick HTL (ASCP) Northwestern University Pathology Core

RE: [Histonet] Frozen section staining line

Lucky for us that cut research frozens and don't have to rush- we stick them on the autostainer starting in water prior to Hemo (we fix in 95% as we cut) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL

RE: [Histonet] staining rat tissue with monoclonal antibody?

We try and find a mouse anti-rat antibody if we can and run rat secondary (Dako makes one) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet

RE: [Histonet] Outside Lab

Is the histo lab in the urology office CAP certified? Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

RE: [Histonet] Blue haze

I'm sure you'll just charm them and they'll never catch on Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED

RE: [Histonet] IHC on paraformaldehyde-fixed

Ruth, I agree with you- As my boss says, they are living in the past. Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: [EMAIL PROTECTED] [mailto

RE: [Histonet] RE: Flame substitute for embedding

We have a Shandon Paratrimmer, which melts the wax not only off blocks but does a good job of keeping those forceps clean and hot. We have a bacti-incinerator, but know we can get a tall flame if we leave the paraffin coated forceps in there!! Bernice Bernice Frederick HTL (ASCP) Northwestern

RE: [Histonet] RA Lamb has been sold to the evil empire

I keep telling everyone it's going to be histosupplies are us one of these days!!! Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court Olson 8-421 Chicago,IL 60611 312-503-3723 -Original Message- From: [EMAIL PROTECTED

RE: [Histonet] Question about Leica Autostainer XL

We have also noticed that we get preciptate and mold in the water drain hose. We bleach it once a month along with the water containers. Our hose is not smooth (corrugated?) Bernice Bernice Frederick HTL (ASCP) Northwestern University Pathology Core Facility ECOGPCO-RL 710 N Fairbanks Court

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