Hi,
I have been asked to do a Sudan stain on a heart biopsy for lipofuscin. The
biopsy is in a paraffin block. They are looking to better report and
understand the IHC. I am totally unfamiliar with this stain. I did some reading
but have been unable to find a protocol for paraffin sections. I
=
________
From: Betsy Molinari via Histonet
Sent: May 5, 2021 9:46 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Movats
Hi Histonetters,
I have received several human vessels for paraffin processing and to stain the
sections for H and Movats. The H were f
ter all, different tissues.
A rather long, and not very helpful reply!
John Kiernan
London, Canada
= = =
____
From: Betsy Molinari via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
Sent: May 5, 2021 9:46 AM
To: histonet@lists.utsouthwestern.edu<mailto:his
Hi Histonetters,
I have received several human vessels for paraffin processing and to stain the
sections for H and Movats. The H were fine. The human sections turned
brownish yellow with the Movats.The control which is canine small intestine was
perfect.
The protocol is standard
Bouins 1hr in
has 20% water. You will still have water in the tissue.
Donna Willis
Anatomic Pathology Manager
Baylor University Medical Center
Baylor Scott Health
214-820-2465 office
____
From: Betsy Molinari via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Wednesday,
5 Discovery Dr Boulder CO 80309
From: Betsy Molinari via Histonet<mailto:histonet@lists.utsouthwestern.edu>
Sent: Wednesday, June 3, 2020 12:50 PM
To: Porter, Amy<mailto:port...@msu.edu>;
'Histonet@lists.utsouthwestern.edu'<mailto:histonet@lists.utsouthwestern.edu>
Subject: Re: [Histon
ado
c/o Kolicko
AES Univ of Colorado
Rm 224, 3775 Discovery Dr
Boulder CO 80309
From: Betsy Molinari via Histonet<mailto:histonet@lists.utsouthwestern.edu>
Sent: Wednesday, June 3, 2020 12:50 PM
To: Porter, Amy<mailto:port...@msu.edu>;
'Histonet@lists.utsouthwestern.edu'<mailto:histone
stored it in isopropyl for a long time I would go down to 70. But it
may not make that much difference.
Shirley
-Original Message-
From: Betsy Molinari via Histonet
Sent: Wednesday, June 3, 2020 2:55 PM
To: Whitaker, Bonnie ;
'Histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet
Hi,
If tissue is fixed in formalin ( I do not know if it is 10% NBF) then put in 80
% isopropanol can the tissue be processed as if it was in ETOH? What are the
effects to the tissue stored in isopropanol?
Thanks,
Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770
-Original Message-
From: Betsy Molinari via Histonet
Sent: Wednesday, June 3, 2020 2:49 PM
To: Porter, Amy ; 'Histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] stored in 80% isopropanol
CAUTION: External Email
I am also in research. I have very little to go on, but I presume
ation for clinical.
____
From: Betsy Molinari via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
Sent: Wednesday, June 3, 2020 2:39 PM
To: 'Histonet@lists.utsouthwestern.edu'
mailto:Histonet@lists.utsouthwestern.edu>>
Subject: [Histonet] stored in 80% isopr
and it makes a mess when deparaffinizing them - that is 1,000 times more wax
than you usually have on a slide!).
Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies Department of
Pathology UC San Francisco Medical Center
-Original Message-
From: Betsy Molinari via
, and permanently delete this e-mail and the attachments, if any,
and destroy any printouts.
-Original Message-
From: Betsy Molinari via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Tuesday, January 21, 2020 7:53 AM
To: Charles Riley; 'Histonet@lists.utsouthwestern.edu'
Subject: Re
st like
you would a paraffin block by putting some fresh paraffin on top of the section
again and allowing it to dry. It would just then need to be reheated and run
down to remove the paraffin cover before staining.
On Tue, Jan 21, 2020 at 6:50 AM Betsy Molinari via Histone
Thank you for the reply Amos. I totally agree but the researcher did not inform
me until after the deparaffinization was finished. She wanted to hold off on
the staining but it was too late and I went ahead with the staining. I was just
curious if there was, in fact a procedure to preserve a
hing without a sharp eye. - Klingon Proverb
On Monday, January 20, 2020, 07:09:59 AM CST, Betsy Molinari via Histonet
mailto:histonet@lists.utsouthwestern.edu>>
wrote:
Good day and Happy Martin Luther King Day. What are your thoughts on letting
deparaffinized slides dry to be used for fu
Good day and Happy Martin Luther King Day. What are your thoughts on letting
deparaffinized slides dry to be used for future use , or keep in DI water for a
time? I deparaffinized some slides this morning, then the researcher called
and asked me to hold off on staining them. I am going to
Hi,
When I place my sections on the waterbath the paraffin pulls away from it.
Leaving just the edge of the tissue. It is very weird. I have tried at
different temps . The tissue itself is fine so I do not believe it is the
processing. I use the same paraffin for processing as well as
earcher to let you try
> something simpler before attempting Heidenhain's AZAN. Wheater's
> Functional Histology has a nice photomicrograph of a section stained
> with H and for endocardial elastin (looks like orcein).
>
> Enough rambling!
> John Kiernan
> Anatomy & C
Betsy Molinari
Sr. Histology Research Technician
CV Pathology Research
Texas Heart Institute
6770 Bertner Avenue, MC 1-283
Houston, TX 77030
Office: 832-355-6524 | Fax: 832-355-6812
Email: bmolin...@texasheart.org
texasheart.org | facebook
| twitter
-Original Message-
From:
Hi histonetters,
I have a researcher that wants to stain Purkinje fibers and has requested a
Mallory-Azan stain.
I have no experience with this stain. I have looked online for information but
am reaching out to you for personal advice.
Thanks.
Betsy Molinari HT,ASCP
Texas Heart Institute
6770
John,
You are such a wealth of information. I look forward to your posts. I learn
something every time. That is going to be my reading list as well.
Thank you.
Betsy Molinari HT (ASCP)
Texas Heart Institute
6770 Bertner Ave
Houston, TX 77030
832-355-6524 (lab)
832-355-6812 (fax)
Betsy Molinari
ette Pavelich, HT(ASCP), QIHC
>
> Sent from my iPhone
>
>> On Apr 26, 2019, at 8:21 AM, Betsy Molinari via Histonet
>> wrote:
>>
>> Hi,
>> I have researched the archives but still have concerns. I have some very
>> small pieces of tissue that are in 70%
Hi,
I have researched the archives but still have concerns. I have some very small
pieces of tissue that are in 70% ETOH. Should I mark them with eosin or
safranin O? From what I have read the tissues were still in formalin, and mine
are already in alcohol. Can they still be marked? At the
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