This sounds absurd to me.
-Original Message-
From: Paula via Histonet
Sent: Friday, July 21, 2023 4:09 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Expiration date after opeing a reagent
External: Be careful with links and files. If suspicious, alert the
Oh, for the days when one could tuck a 32 oz. Slurpy inconspicuously behind the
VIP without fear of admonishment.
-Original Message-
From: Jay Lundgren via Histonet
Sent: Friday, June 2, 2023 3:34 PM
To: Piche, Jessica
Cc: Histonet@lists.utsouthwestern.edu
Subject: [External] Re:
We use an Azure B counterstain. Melanin has a greenish tinge after
counterstaining.
-Original Message-
From: jayalakshmy p.s via Histonet
Sent: Monday, April 11, 2022 11:59 PM
To: histonet@lists.utsouthwestern.edu
Subject: [External] [Histonet] Clarification about Immunohistochemistry
Is there any other way! :-O
Sent from my iPhone
> On Feb 28, 2019, at 3:30 PM, Mark Tarango via Histonet
> wrote:
>
> I hope everyone is using on-slide controls :-)
>
> On Thu, Feb 28, 2019 at 11:52 AM Terri Braud via Histonet <
> histonet@lists.utsouthwestern.edu> wrote:
>
>> Another
Is anyone running NOTCH2 on Bond III OR Benchmark Ultra? Can I take a peek at
your protocol and antibody selection?
Sent from my iPhone
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There are slide basket inserts to accommodate that sized slide for Sakura
stainers. The DRS2000 for example. Check out this web page.
http://www.tedpella.com/histo_html/slides-large.htm
-Original Message-
From: Atoska Gentry via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Hi Mark,
We still have this issue after two years of complaining to Roche. We've had
multiple TAS's come in and we've sent slides to Tucson. They've gone over the
instruments, etc. Long story short, their fix was adding something to their
package insert that says "Schiff's Reagent is known to
I'm curious as to why we heat the Auramine Rhodamine when we are staining
tissue sections but our Micro lab stains at room temp. It is because "it's
always been done that way"?
Thanks group,
Angie
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This seems to be the trend of society in general these days.
No manners.
-Original Message-
From: Hudson, Pamela R ASHVAMC via Histonet
[mailto:histonet@lists.utsouthwestern.edu]
Sent: Wednesday, May 04, 2016 7:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW:
Hi Histonet Colleagues,
I'm surveying Histology labs about Auramine-Rhodamine staining for acid fast
organisms.
If you'd like to help me by participating in my survey please follow the link
below.
Thank you in advance for your responses.
https://www.surveymonkey.com/r/7JPL5K2
Angie
Angela
Has anyone been able to optimize an antibody to BCL-10 using Ventana's
Ultraview detection?
Angie
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Is it me or does anyone else think $20 is a lot of money for a T-shirt? The
Histotechnology Professionals Day T-shirts on NSH'S website seem over-priced.
Angie
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to it, if any) is confidential and may be legally
Can anyone tell me what kind of glycogen to use for this? Sigma's website has
multiple choices. Thanks.
Angie
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to it, if any) is confidential and may be legally privileged. It is intended
solely for the addressee.
For anyone out there still using the old microtome knives that you have to
sharpen yourself- I have a box full, maybe 20 knives that are free to a good
home. Contact me off line if you are interested.
Warmest regards,
Angie
Angela K. Bitting, HT(ASCP),QIHC
Technical Specialist for Special
What does this group feel is an acceptable repeat rate for automated IHC
staining??
Thanks for your responses in advance.
Angie
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Does anyone have any experience with using these slides on a Ventana platform?
Thanks in advance,
Angie
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So sad bc spider plants THRIVE ON the fumes!!
-Original Message-
From: Michelle Lamphere [mailto:michelle.lamph...@childrens.com]
Sent: Sunday, May 31, 2015 8:36 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] plants in the lab
Our hospital Safety and Infection
Good morning Histonetters,
Is anyone else having issues with their Ventana detection? My dispensers are
clogging up with rubbery plugs and mis-dispensing. It's not that they sit in
the refrigerator and don't get used for long periods of time. We are a high
volume lab and we go through
Does anyone know of a way to generate a visual alert (hearing impaired device?)
to notify us when our processors finish?
They are going to be elsewhere in the building and we need to know when they
finish. Baby monitor is out of the question due to privacy issues.
Sent from my iPhone
Can anyone recommend a third party auto dialer to connect to Peloris?
Thanks,
Angie
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We occasionally see this also.
Sent from my iPhone
On Apr 21, 2015, at 7:59 PM, Sue suetp...@comcast.net wrote:
OMG we are experiencing the same issue. At first it was just GI and now we
are seeing it on prostate. One pathologist said it looks like the tissue has
been cooked. The only
We just tried a moldy strawberry. It was loaded with fungus!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Piche, Jessica
Sent: Friday, April 17, 2015 7:11 AM
To: 'T Williams';
Can anyone recommend a good dishwasher for cleaning my special stains glassware?
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Has anyone had any luck optimizing DPC4 aka SMAD4 with Ventana's XT or
BenchmarkULtra platform?
Any help is appreciated.
Angie
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to it, if any) is confidential and may be legally privileged. It is intended
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Hello Fellow Histonetters,
We often get slides from another hospital for Oil Red O staining. On occasion,
they send them at room temp. and until they get to us it's been a day or more.
I'm worried that because they are unfixed the cells will be autolyzed and I
don't want to charge the patient
, 2015 5:20 PM
To: Bitting, Angela K.
Subject: Re: [Histonet] CBG Recycler users
Hi Angie, I used it for quite a while, what's up?
Michelle
Sent from my iPhone
On Feb 10, 2015, at 10:40 AM, Bitting, Angela K. akbitt...@geisinger.edu
wrote:
Would someone who is a CGB alcohol recycler user contact
Would someone who is a CGB alcohol recycler user contact me? I can't get
through to CBG.
Thanks,
Angie
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I'm looking for another institution who is running Ventana's MDM2 DNP probe. If
you would be so kind as to contact me.
Thanks,
Angie
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Thank you everyone for your swift replies.
-Original Message-
From: STACEY [mailto:stace...@sbcglobal.net]
Sent: Monday, February 02, 2015 3:57 PM
To: Bitting, Angela K.; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Benchmark Special Stainers
Yes, it is the same. (Carboy
Can anyone tell me if the waste collection on the Benchmark Special Stainer is
the same as the waste collection on the Benchmark Ultra or XT? Carboy with
spigot??
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We are being inspected soon and I'm trying to check the labels on all of my
chemicals. How do I determine the expiration date of a 1N HCl solution I
prepared in house. I went on line and a commercially available 1N HCl solution
has a 36 month shelf life according to the vendor's web site. I
We also have the Grand Master. It's quite amazing, however we have had issues
with the punches occasionally. Also alignment issues which turned out to be
software related. Have you seen any of this Ashley??
Regards,
Angie
Sent from my iPhone
On Dec 2, 2014, at 8:59 AM, Troutman, Kenneth A
Yes, it's part of the requirements to be able to take the exam.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amy Johnson
Sent: Wednesday, October 29, 2014 12:19 PM
To:
ditto
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Thursday, October 30, 2014 3:45 PM
To: madeathri...@pastnashville.com; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet]
Our new Pathologist would like to see more yellow in the background of our Gram
stain. We use picric acid-acetone currently, which looks more pink than yellow
most times. So I'm wondering if a method using a Tartrazine counterstain would
be better.
Any thoughts?
IMPORTANT WARNING: The
Does anyone have something that I can show to nursing to explain why a
microtome laceration is not an exposure? I can't get the fixed tissue
explanation to sink in. Anyone else had issues with this?
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if
Is anyone else in Histoland having issues with the Ventana PAS kits?
Particularly, crystals laying on top of the tissue after staining. If so,
please contact me off line.
Thanks,
Angie
Angela K. Bitting, HT(ASCP),QIHC
Technical Specialist for Special Stains/ IHC/ISH
Geisinger Health System
100
28 years here
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Howery, Jeff L
Sent: Thursday, August 07, 2014 3:54 PM
To: 'Paula Pierce'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] On the
Ventana Benchmark Ultra users: Where are you buying your Desmoglein-3? I need
an IVD product.
Thanks in advance,
Angie
IMPORTANT WARNING: The information in this message (and the documents attached
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solely for
We used to use a pc. Of proliferative endometrium, a pc of secretory
emdometrium and a pc of cervix in a multitissue block for our ER/PgR control.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Barbara
If anyone is currently using this processor I'd love to hear some feedback on
this specific model.
Thanks,
Angie
IMPORTANT WARNING: The information in this message (and the documents attached
to it, if any) is confidential and may be legally privileged. It is intended
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Our facility handles paraffin shavings and blocks as Biological Waste which is
different from Biohazardous waste.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM DESALVO
Sent: Thursday, July 10,
I posted about this earlier this year. Apparently they are no longer available
in the US. I have found no substitute for these racks or adapter and Sakura was
unwilling to help in any way. (Which I am still miffed about).
If someone has a metal shop, here is a great opportunity.
Angela K.
FITCs have to be purchased directly from Ventana. I've been trying to figure
out how to trick their system because I have third partys that I'd like to
try out. They don't offer an option to choose a Prep kit in their FITC
template. When I called in to ask why they don't allow it they told me
I think it's pretty standard that outside institutions put their accession ID
on the front of the consult slides.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amber McKenzie
Sent: Wednesday, March 19,
www.drsfostersmith.com
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Tighe
Sent: Friday, January 24, 2014 10:51 AM
To: histonet@lists.utsouthwestern.edu (histonet@lists.utsouthwestern.edu)
Subject:
So Mike,
It appears you are a sales director for the company, which makes your opinion
null and void. Sorry.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Thompson
Sent: Thursday, January 23, 2014
We had one screen replaced early on but no problems since. However, we've had
to replace the plastic lids several times and the parts are always on back
order. The one that covers the molds breaks right off.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We track shallow/incomplete sections and have found numerous reasons.
Inadequate fixation.
The way a tissue is bisected can cause it to not lay flat in the embedding
mold. For anstance a grossing tech who does a wrist turn kind of slicing
motion and causes an arch shaped edge.
Embedding
That should be the slogan for NSH next year :D
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda
Sent: Tuesday, January 07, 2014 11:26 AM
To: Rathborne, Toni; 'Eytalis, Robert A'; Anne Murvosh;
That's my understanding as well.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mark Tarango
Sent: Monday, January 06, 2014 6:03 PM
To: Johns, Jill
Cc: histonet@lists.utsouthwestern.edu
Subject: Re:
The article doesn't even mention the repetitive motion injuries.
IMPORTANT WARNING: The information in this message (and the documents attached
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Yeah, wow, I just read through this thread I guess haters are gonna hate.
Nobody's got time for that.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of hymclab
Sent: Wednesday, September 04, 2013 12:55
If you keep the layer of paraffin thin enough, it can still be scanned with
most barcode scanners.
-Original Message-
From: Blazek, Linda [mailto:lbla...@digestivespecialists.com]
Sent: Thursday, August 29, 2013 11:15 AM
To: Bitting, Angela K.; Morken, Timothy; Histonet
Subject: RE
I am using a polyclonal antibody to B. burgdorferi. Item #BP1002 from Acris
Antibodies.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jan Shivers
Sent: Wednesday, August 14, 2013 1:39 PM
To: Delray
I saw a few a while back. Pretty funny. It looks like they were made by some
Pathology residents and techs.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Sanders,
Jeanine (CDC/OID/NCEZID)
Sent:
I believe so, yes. This is the info I was given. Buy Biotin Antibody Rabbit
Polyclonal: A150-109A from Bethyl Laboratories, Inc. 25043 West FM 1097,
Montgomery, TX 77356, 1-800-338-9579
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Something to remember is that humidity is the enemy too. If you are storing
your slides in a refrigerator make sure that the humidity is well controlled.
We found out the hard way.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Vikki, Didn't they tell you?? You were voted off the island.
:)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Victoria Baker
Sent: Tuesday, April 23, 2013 2:10 PM
To: Histo Net list server
Subject:
Technically, he would be right.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tim Higgins
Sent: Tuesday, March 26, 2013 10:18 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Batch
We hate Leica's CV5030 coverslipper too.!!! Babysit is a good word to
describe the user experience.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver
Sent: Wednesday, March 20, 2013 7:32 PM
I was given info from a Dako rep last week that if the primary antibodies are
applied separately to the slide as is true with Ventana instruments, you can
charge for both. I am skeptical. Has anyone else heard this?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Just spoke with my Ventana rep about this same topic. My understanding is ANY
vendor who sells HPV probe for ISH is out of compliance with FDA and is
eventually going to get in trouble. I specifically asked if I could buy probe
from elsewhere and was told not likely. This is only a problem for
I just tried them Friday. They worked fine but the label picked up counterstain
a bit. However, depending on the cost per label, it may be worth the trouble.
I'm waiting for a quote.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Are you using the antibody from Dianova??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Zimmerman,
Billie
Sent: Friday, February 22, 2013 4:21 PM
To: histonet@lists.utsouthwestern.edu
Subject:
I received a spec sheet from Dianova that has their Ventana XT protocol. It
suggests using CC2 standard retrieval and incubation for 32 minutes with amp.
They give a dilution range of 1:20-1:50. It seems to work well for us although
we are still validating. I hope that helps.
-Original
Well, if it's more than 2.5 sq. ft per tech, we're out of compliance :)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
jmasla...@stpetes.org
Sent: Monday, December 10, 2012 12:41 PM
To:
Histonet should stay free of those entanglements(political opinions)
Funny, when I wrote that here 2 weeks before the election no one wanted to hear
it.
What has changed??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Are we a bunch of catty 13 year olds or can we stick to Healthcare?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Davide Costanzo
Sent: Tuesday, November 20, 2012 10:31 AM
To: Rene J Buesa
Cc: Fellow
Here we go with the political opinions again. They have no place on this forum.
It's bad enough that Facebook is polluted with them.
Have a nice day!
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene
I'm going back to Facebook now. Let me know when this thread poops out.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Hermina
Borgerink
Sent: Wednesday, October 31, 2012 11:33 AM
To: Rene J Buesa; Kaye
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