[Histonet] electron microscopy on bone

I'm hoping someone might be able to guide me to a laboratory which is capable of completing transmission electron microscopy on cortical bone or perhaps would be willing to share a procedure. Our interest is in osteoclast ultrastructure. Thank you. Eric A. Breisch, Ph.D. Clinical Anatomist

[Histonet] SV-40 control tissue

Does anyone have a commercial source for SV-40 control tissue to be used in IMPX? Thanks for your help. EAB Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital San Diego Associate Clinical Professor of Anatomy Dept. of Surgery/Div. of Anatomy UCSD School

[Histonet] protein concentration for Cy2

This question if for anyone doing C4d immunoflourescence staining. Could someone share what protein concentration the Cy2 labeled anti-mouse IgG is prepared at and what buffer is used for that? Our final dilution of the Cy2 is (1:100 in 1%BSA/PBS) but we don't know what the original protein

[Histonet] cross contamination

Hi Histonetters- Our laboratory does not process gynecologic cytology specimens and we have a relatively small number of non gynecologic specimens. Usually when we process the non gynecologic cytology specimens, they are done separately. I saw a CAP question which addresses prevention of cross

[Histonet] NADPH-diaphorase

Hi, hoping that someone might be able to share with us where they purchase NADPH-diaphorase for the NADPH stain. Thank you. EAB Eric A. Breisch, Ph.D. Clinical Anatomist Dept. of Pathology Rady Children's Hospital and Health Center Associate Clinical Professor of Anatomy

[Histonet] bone histology and storage

Does anyone have a methodology for long term storage of bone? Formalin vs alcohol? Orthopedic group here is wondering whether bone for research purposes should be preferentially stored in alcohol. They aren't interested in immunohistochemistry but rather tensile properties of bone. I haven't been