Re: [Histonet] What is the best xylene substitute for histology?

As usual, great insights/advice from JK I always learn from you, Sir You are THE person that I "listen" to ( you may have stood on the shoulders of others?? Do tell) I'm always looking for xylene substitutes.haven't found any as efficientsigh I run the gamut with my Safety people

Re: [Histonet] Alcohol

For 50 yrs I have used Industrial methylated spirit ( 74OP IMS) It is cheaper than ethyl alcoholdoes the same job as it is ethyl alcohol containing a part of methyl alcohol No duty charged Until recently I would buy 25 L drum Carl Hobbs FIBMS Histology and Imaging Manager Wolfson SPaRC Guys

Re: [Histonet] [EXT] Reagent alcohols and tissue

I ask Researchers to give me cassettes in 90% alcohol as I start my "dipN dunk" processor ( Leica TP 1020) at 90% They fix, rinse in tap water, dist water, x2 70% alcohol, then bring to me in 90% alcohol This way, less likely to contaminate my 90% alc with their inadequate alcohol rinses. Been

Re: [Histonet] tissue cassettes

Why would anyone use a different wax for infiltrating and embedding? Yeskeep them specimens molten until embedded Thanks for your input, Paula Time flies like an arrow Fruit flies like a banana BonBon-illy Carl ___ Histonet mailing list

Re: [Histonet] tissue cassettes

I'm interested but don't understand the variation and it's + effect I take my cassettes out of the processor and immediately place into the molten wax bath of the embedder ( if I'm embedding immediately; if not I let the cassettes/tissues therein go cold until a later embedding) When embedding

Re: [Histonet] DAB freezing (Alonso Mart?nez Canabal)

I do manual IHC-DAB regularly DAB shouldn't "go off" after freezing unless you have somehow oxidised it during mixing/aliquoting. It would then show as a dark brown aliquot I state this because I still make up my own DAB from dry powder ( have been doing for > 20yrs) SIgma D5637 I dissolve,

Re: [Histonet] IF staining questions (Charles Riley)

The primary antibody is the most expensive reagent If you use the primary ab unconjugated and visualise using a conjugated secondary you will significantly increase the dilution factor of the primary ab thus your primary ab will last much longer ( saving you money..and, maybe less

Re: [Histonet] maximum thickness brain in slide

Hi Alonso Firstly, what antibody( ies) are you wanting to use on your Pwax sections? If you are looking just to identify proteins, 8 microns is sufficient I mount my brain sections ( human, ms, rat, axolotl, worm, fruitfly, pig...etc) then airdry them O/N in a working fume hood Then, before