Hi Alonso Firstly, what antibody( ies) are you wanting to use on your Pwax sections? If you are looking just to identify proteins, 8 microns is sufficient I mount my brain sections ( human, ms, rat, axolotl, worm, fruitfly, pig...etc) then airdry them O/N in a working fume hood Then, before dewaxing, I put them into a 60C oven for 1hr.
Imho, free-floating sections are useful only if you wish to study projections over depth Use Superfrost Plus ( or equivilent) slides Good luck! Carl Carl Hobbs FIBMS Histology and Imaging Manager Wolfson CARD Guys Campus, London Bridge Kings College London London SE1 1UL 020 7848 6810 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
