It must be completed in seconds. Buried in powdered dry ice is one way.
Immersion in a slurry of dry ice and isopentane is another.
Cordially,
Charles W. Scouten, Ph.D
Product Specialist, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
Unit
The blade angle should always be so the final bevel face on the bottom
of the blade is parallel to the direction of motion. This is discussed
in Dr. Peter’s book, “A Practical guide to frozen sections”
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystem
Carve a channel in balsa wood, available at hobby shops, and sandwich
the otolith in. Section wood and all.
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL 60071
United States of America
Teleph
Cracks and fissues is not freezing artifact, as from freezing too slow.
You are cutting the tissue too cold, and/or the blade angle is too
steep.
Cordially,
Charles W. Scouten, Ph.D
Product Manager, MNL
Biosystems Division
Leica Biosystems Richmond, Inc.
5205 Route 12
P.O. Box 528
Richmond, IL
I wonder what pressure you were working at? You can't tell if you
control flow rate. It may have been very high.
Certainly, it would be possible to put the pressure high enough to
produce all the problems you describe, and fluid would come out the nose
as a part of that. But how high is that
I have perfused mice and rats at 300 mm Hg, about double physiological
level, don't know what that made the flow rate. All mammals have the
same blood pressure (within tolerances), so it is easier to select a
suitable pressure to use than a flow rate, which varies dramatically.
I look at bra
It is not a "calibration" issue. Most likely cause it too shallow a blade
angle, another is something is loose and wobbly. Try grasping the blade holder
and see if it can be moved in any direction. The tissue pedestal. If
something is loose, that is the cause.
Cordially,
Charles W. Scouten,
