[Histonet] Thermo IHC

Hi Histonetters! I need some help. We just acquired a Thermo IHC system. Can anyone help us cut through the *88*888 and give me some helpful tips? We have practical experience on the Ventana systems only. Has anyone been successful at using only 1 HIER buffer? Any tips on how to shorten the

[Histonet] Shandon Excelsior ES®

What do people think about the Shandon Excelsior processor? I currently have the old Miles-Sakura VIP and we love it but we are looking for another processor so we can have 2 processors for fatty tissue. I am ok with getting another refurbished VIP. I have excellent service contracted with a Bi

[Histonet] FW: Thermo-scientific vs Ventana Benchmark XT

Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 From: Cheri Miller Sent: Monday, September 08, 2014 10:17 AM To: histonet-boun

[Histonet] FW: Me again

From: Cheri Miller Sent: Monday, September 08, 2014 10:31 AM To: histonet-boun...@lists.utsouthwestern.edu Subject: Me again What are peoples thoughts on the Thermo Scientific Excelsior AS processor? Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene

[Histonet] Sausage blocks and tissue arrays

What is everyone doing for IHC control tissue? My path that made my sausage blocks is no longer here and reality has kicked in! I need to find a suitable replacement. Is anyone making their own control block or "Sausage" without using the microarray punch and paraffin block? Thanks, Cheri Che

[Histonet] Fungus control

Hello wise ones! I am having an issue with yeast floating off my lab, agar grown fungus controls. Micro made us a beautiful control 5 months ago. Now all of a sudden we are seeing a significant amount of yeast floating off the control and attaching to the outer edges of the patient tissue. I t

[Histonet] RE: New GHS labels

68117 402 731 4145 ext. 532 Cell 402 493 0403 Fax 402 731 8653 From: Stacy McLaughlin [stacy_mclaugh...@cooley-dickinson.org] Sent: Thursday, February 27, 2014 1:59 PM To: Cheri Miller; histonet@lists.utsouthwestern.edu; histonet-boun...@lists.utsouthwester

[Histonet] New GHS labels

I'm getting ready for my CAP inspection and I came along information about the new Global warning labels that were to replace the NFPA labels we currently use for our chemicals. Is everyone using these or can we still use the NFPA label and placards? I remember hearing about it at a seminar just

RE: [Histonet] RE: Negative Controls for IHC

I just stopped using the reagent controls on all our IHC due to the drastic cuts in reimbursement. It will be a significant immediate savings for our tiny lab. Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4840 F St. Omaha , NE. 68117 402 731 4145 ex

RE: [Histonet] Microtomes

Leica always Cheryl A. Miller HT ASCP cm Histology Supervisor Hygiene Officer Physicians Laboratory, P.C. 4140 F St. Omaha , NE. 68117 402 731 4145 ext. 532 Cell 402 493 0403 From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthw

[Histonet] Aldex or aldehyde neutralizer spray

Does anyone know where I can locate an Aldehyde Neutralizing spray? We would spray it on paperwork that had a leaking specimen container to neutralize the formalin that spilled onto the requisition. Cheri Cheryl A. Miller HT ASCP cm Histology Supervisor Physicians Laboratory, P.C. 402 493 0403

[Histonet] RE: Waterbaths

I agree with you. Ours seem to short out as well. I now only purchase the baths that have a separate Pyrex glass insert. They also provide more of an "edge" space to lay out your ribbons when cutting multiple levels and ribbons. Cheri "March 10, 2013 is Histotechnology Professionals Day" Cheryl

[Histonet] Fungus Control Tissue

I need a new Fungus control block. Can anyone spare some? Thanks. Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor, Hygiene Officer Physicians Laboratory Services 4840 F Street Omaha, NE. 68127-0999 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATI

RE: [Histonet] Basis for Quality Work in a Histotech

I totally agree with you Rene. May I add as a supervisor of 6 in which all of them I trained all myself in an OJT situation. 1. Instill in them team work and pride in their profession 2. Reiterate to them that their unique skills are an important link in the health care chain. If they lack any

[Histonet] Charges for cutting research case

I would like some direction in regards to how to charge a client for cutting multiple slides and levels anywhere between 10 to 50 slides per block of a Technical component case. These would be research cases. Any help directing me would be greatly appreciated. Thank, Cheri Cheryl A. Miller HT(

[Histonet] Fungus , GMS Aspergillus

I am looking for natural occurring Fungus, GMS Aspergillus to use as my fungus control tissue. Someone from Histonet gave me a block about 3 years ago. I've just used the last of it. Does anyone have any to share again? Thanks Cheri Cheryl A. Miller HT(ASCP)cm Histology Supervisor Physicians La

[Histonet] grossing tech

I have a grossing position open, I have a non certified. Non degreed awesome tech with 27 years. Anyway I can have her Gran-fathered in by CAP regulations? I'm pretty sure I know the answer I am hoping to get around this, Cheri Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physi

[Histonet] Congo red

Anyone have any solution to a week Congo Red? I use Rowley Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol diffe

RE: [Histonet] HT Exam

Congrats!! I still remember that day, I cried and my husband thought I was crying because I didn't pass when I was crying from pure joy and relief! Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Messa

[Histonet] (no subject)

How many labs have data entry people assigning surgical case numbers in their processing departments? Basically how many histology departments have AP surgicals processed like clinical specimens? Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha,

RE: [Histonet] IHC run print outs

I submit them with the slides, The Dr will put his interpretation and then I keep them with my QA for 2+ years. Cheryl A. Miller HT(ASCP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained

[Histonet] RE: Off-Topic: April Fool's Day

We got a large specimen container, ones we use for mastectomies and colons (specimens the paths would need to gross in. We labeled it as a normal specimen, put a large piece of foam rubber inside and then put a rubber Rat inside. When the path opened it the foam rubber propelled the rat out of t

[Histonet] looking for KJ Kellogg

I am looking for KJ Kellogg, or Kjirsten Kellogg she is a HT/PA ASCP working some where in Kansas I think. KJ is originally from Wisconsin. I have an urgent, personal message for her. Thanks, Cheri Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Labora

RE: [Histonet] batching controls

We run 1 control per antibody within a case, so if we had 3 mart-1 on 3 separate cases I would run a control for each case Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor, Safety Officer, Physicians Laboratory Services Omaha, NE. 68127 402 731 4145 ext. 554 -Original Message-

[Histonet] RE: Plain Vanilla Autostainer?

I will second that! Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of susan.wal.

RE: [Histonet] Advanced Stainers

I really have nothing bad to say about the benchmark. We love ours; it has freed us from having to nurse the IHC bench. You load it and walk away. If there was a down side I would say that the antibodies are a bit on the higher price side. You can find cheaper antibodies but then you have to us

[Histonet] RE: Acceptable way to "bake" sections onto slides?

Better an extra step than inadequate baking. We put ours in an oven for 20 mins, cool with a fan and then put on our auto stainer. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histon

RE: [Histonet] (no subject)

He did not meet his and his employers agreed stipulations for employment. If he was being paid equal to that of a registered tech then he should receive a decrease in pay. If not then he should continue with merit increases only. Getting an ASCP registry is a marketable achievement for both the

RE: [Histonet] Reprocess

[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller Sent: Wednesday, July 07, 2010 10:53 AM To: sgoe...@xbiotech.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Reprocess Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run

RE: [Histonet] Reprocess

Hi Sarah, I don't melt them down I just drop them in with my dirty molds and lids and run them through the cleaning cycle on my processor. The Xylene and Alcohol will melt the oily fat. When the clean cycle is complete I just drop them back into formalin until I process that evening. It works ve

RE: [Histonet] H&E slide cost

There are so many variables, it all depends on your operating costs i.e. Labor, reagents, maintenance contracts and capitol equipment if applicable. I just completed my cost analysis today and it costs my department approximately $8.15 to produce 1 H&E slide, $2.55 each Special and $38.71 for ea

RE: [Histonet] Re: Grossing Technician Qualifications

That is my understanding as well. Cheryl A. Miller HT(ASAP)cm Histology/Cytology Prep Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4145 ext. 554 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf

[Histonet] (no subject)

Is anyone using Surgipath's Select Tech H&E kit? I have a sample kit and I want to try it out on my Leica XL autostainer. I am looking for a protocol with times/dips etc. Thanks, Cheryl A. Miller HT ASCPcm Histology Supervisor Physicians Laboratory, P.C. 402 493 0403 __

RE: [Histonet] ascp exam

It is that difficult. I studied for months and months. While I passed the first time there were some difficult questions. I felt it was because a good part of what I needed to know I had no practical experience to draw from. Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Ser

[Histonet] charge for pin 4 cocktail

What are people charging Pin 4 p63,CK5-14and P504S..88342 x 3 or 4 Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated

[Histonet] RE: Traveling Histo Tech

Only to say I am envious, good luck and have fun! Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible

[Histonet] FW: New CAP grossing guidelines

That would be my guess; I have on file copies of degree and course study for my grossing personnel Cheryl Miller HT ASCP cm Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-b

FW: [Histonet] cassette labels erased by processor

YES! That is why I use pencil only. I had a processor full of blank/ unlabeled cassettes because the lot# of the pens was bad. There is no way of knowing if the ink is reagent proof until a disaster like you have occurs. You don't always know if the ink lot passed its QC with absolute certain

[Histonet] looking for employment

I am inquiring about employment for my oldest daughter. She is looking for a part time 20 + hours lab assistant position in the Omaha area. She is relocating from CA. She is working on her BSRN and can be flexible with her schedule. The earlier the hours the better. She has some knowledge of His

RE: [Histonet] Re: Diff-Quik

Every slide I stain, special stains, IHC or otherwise I check under the scope...I have taught all my techs to do the same, other than batches of H&E and then we check the 1st slide in each rack. I know this to be a common procedure with many histology professionals. The attitude can be left in

[Histonet] re;Biomedical repair

Thanks to all of you who responded. I had no idea I would catch so many peoples attention, especially Vendors (yikes). I will think twice before I send another one for sure. I have heard good things about Hawkeye Biomedical from many of you and they are currently working with me on getting what

RE: [Histonet] tissue retention times

We do the same. We aren't a MOHs operation but we do many frozen cases and we process the tissue, cut a permanent processed slide of the frozen sections and then retain them for 10 years along with our other blocks. Cheri Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Service

[Histonet] Reliable Bio repair/ refurbish contact in the Omaha area

I am in need of a Reliable Bio repair/ refurbish contact in the Omaha area. I am less than satisfied with my current one, I have a 15 year history with them, but sadly I question their quality and integrity. The poor service is so blatant it is now become a reflection of me in my superior's eyes

[Histonet] CAP regs

Does CAP require an ASCP certification for a supervisor or manager to run a lab or would a BA in science be accepted. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If

[Histonet] re; eosin

Thanks for all your help. It makes sense that it would evaporate faster on the Leica as opposed to hand staining. The eosin on the Leica is uncovered for 6 plus hours a day when hand staining it was covered except when we were staining a rack. I will add 100 alcohol as needed. Again thanks for a

[Histonet] re; eosin

I have had some great suggestions from many of you, the problem is I am doing literally all of them. My question is on the Leica XL isn't the instrument supposed to vibrate and shake a bit to rid of excess fluid?? Maybe that's why it is keeping a lot of carryover because it isn't vibrating. Als

RE: [Histonet] RE: Eosin evaporation.

,Janice A; Cheri Miller; histonet; histonet-boun...@lists.utsouthwestern.edu Subject: RE: [Histonet] RE: Eosin evaporation. I agree Sally; we cover as soon as the run is over and had to adjust the level of our H & E since the frosted end of the slides were getting stained as well. We also tr

[Histonet] Eosin evaporation.

I recently purchased a leica refurbished autostainer, Before we hand stained our by hand. Now I notice the eosin is getting very low very fast, we have to replenish almost everyday. Before a bottle of Richard Allen Eosin would last the week or at least six days. Any idea's? Cheryl Miller HT ASC

RE: [Histonet] Injuries in the Lab

We send them to an urgent care facility under workmans comp. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behal

RE: [Histonet] Leica vs Ventana

Why the switch? I am a big fan of Ventana. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for del

[Histonet] RE: slide handling for special stains

What would be the point?? Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Charles, Roger Sent: Monday, De

[Histonet] RE: one more time. This is what I meant to send

Once more and I will put it to bed. No I am not an old tech... I have been in histology for 18 years. May I quote Dr. Mohs " Only a histotech can produce something so beautiful and useful as a slide" ...sliding off my soapbox . Cheryl Miller HT ASCP CM Histology Supervisor Physicians Labo

[Histonet] one more time. This is what I meant to send

Am I the only one offended by this ?? Thank goodness for his co-workers, He will be able to rescue them from their plightRemember when histology was in a dark dank room next to the morgue?? Show some respect for those elderly minimally competent techs ... Most of them taught themselves

RE: [Histonet] what book to study for registry?

Am I the only one offended by this ?? I'm working with a thirtysomething man with a degree in chemistry and a good bit of experience under his belt, who in these bad times wound up signing on as a histech with no experience. Trained by an elderly minimally competent tech, he's rapidly bl

[Histonet] mistake

Sorry didn't mean to resend, guess it's a Friday thing?? Maybe not. Cheryl Miller HT ASCP CM Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or

[Histonet] RE: Laboratory Licensing

I agree with you, what happens when a lab processes only? We in the past have had accounts that we would gross, section, stain and coverslip. We then send completed slides to the submitting Physician usually a Dermatologist who likes to read and diagnose his own slides. Cheryl Miller HT ASCP CM

RE: [Histonet] Returning Placentas to Patients

Smart!! Cheryl Miller HT ASCP Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun Sent: Friday, October 30, 2009 12:29

[Histonet] (no subject)

test Cheryl Miller HT ASCP Histology Supervisor Physicians Laboratory Services Omaha, NE. 402 731 4148 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intended / indicated or agent responsible for delivering it to the addressee, you are her

RE: [Histonet] AAVLD Histo sample block & slide retention policy

I disagree, the slide is what was used to make the diagnosis. The block may not be a good representation of the actual slide, it may could have been cut so that what is on the original slide is no longer in the block. In Surgical path we keep our slides indefinitely and our blocks for 10 years.

[Histonet] RE: Special stains and microwave

We had one and we really didn't use it often enough to justify the cost. Maybe 2 or 3 stains benefited from it, otherwise waterbath and incubators were the preferred method for most of us. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -Origin

[Histonet] refrigerating sliver and gold chloride??

I just noticed that my silver and gold chloride says to store at room temp now. I have always stored them in the refrigerator. Is anyone still doing this?? Old habits die hard. I want to know if this has caused any staining issues for anyone. Cheryl Miller HT (ASCP) Histology Supervisor Physici

[Histonet] Saturday opening

I have an opening for a part time HT working Saturday mornings only (approximately 5-8 hours) anyone interested contact me. Thanks Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INF

[Histonet] IHC validations

Does anyone have a validation procedure on approved protocols for your IHC? I have a new Ventana Benchmark XT. I have a validation policy and procedure for clinical but it doesn't relate well to IHC. In the past I have written on the protocol and run procedures the date it was approved by a path

RE: [Histonet] Nice one Kemlo

I for one would be lost with out this group. I need and miss the collaboration of fellow histotechs on a daily basis. Cheri -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of alan taylor Sent: Saturday, April

[Histonet] Mesothelioma control tissue

Hi Histonetter's, I am in need of a mesothelioma control block for my IHC. Is there anyone that would be willing to share? I might have tissue that you may need as well. Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 50

RE: [Histonet] metal molds vs. disposable molds

My issue with the plastic disposable molds is that when you need to re-embed and melt the block down, the mold will curl and lose its shape just enough to impede cutting the second time around. Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 -Or

RE: [Histonet] Practical Exam, RE: testing cutting ability

Where is the fence??? It looks like a P---ing match between dogs. We ALL worked hard on our certification no matter how we got it. And due to the shortage I assume we ALL work hard now. Fast or slow matters not. Histology, good histology needs many talents such as; scientific knowledge, troublesho

RE: [Histonet] uncertified techs in Histology

Judy, Judy, You are exactly on the right track! I remember when I was training I worked with several techs. many shared their expertise, tricks and the little nuances we all have to make that tissue cut better or the stain to 'pop' They made me do the troubleshooting with gentle prodding, quizzin

[Histonet] OJT Uncertified techs

I can't find Certified techs to work in my lab. I have 1 registered tech close to retirement, 1 non-certified tech with 30+ years in histology and she is limited to microtomy only, and me. I have 2 OJT techs that are really quite good. I am teaching them everything I know, making them look at thei

RE: [Histonet] Work flow, quality issues

What is the gain??? I am confused by their methods. I also face(rough cut) and section on the same microtome. I noticed over the years that cuts on the same microtome and block, one tech can get a thin section just by the way they turn the flywheel. A heavy handed cutter can make the section thicke

[Histonet] Aspyra HELP!!

Does anyone out in histoland have the Aspyra -Cyberpath computer system?? We are currently setting ours up and I need some serious help. I prefer not to say it here so I wont be flamed by a representative of the company. PLEASE HELP! Cheryl Miller HT (ASCP) Histology Supervisor Physicians Labor

[Histonet] Amyloid control tissue

Does anyone have any Amyloid tissue they are willing to share?? Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be contained in this message. If you are not the addressee intende

[Histonet] Histology Positions

Is there anyone looking for a Histotech or a Histology Supervisor in the Omaha, Council Bluffs or Lincoln area?? Let me know. Thanks, Cheri Cheryl Miller HT (ASCP) Histology Supervisor Physicians Laboratory,P.C. Omaha, Ne. 402 738 5052 PRIVILEGED / CONFIDENTIAL INFORMATION may be