We have seen surface tension issues with staining on slides. We had several
lots and were able to remove the bad lots from our stock and that has helped.
Cindi Robinson HT(ASCP)
Histology Lead
MercyOne Siouxland Medical Center
Dunes Medical Laboratories
101 Tower Road, Suite 220
Dakota Dune
We use safranin at the grossing station and it is a dark pink at embedding.
Works really well in our hands. Added plus is no fluorescent issues that you
can have with eosin.
Cindi
From: Cindy Bird via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Th
Aren't fs CPT codes 88331 and 88332?
From: Terri Braud via Histonet [histonet@lists.utsouthwestern.edu]
Sent: Friday, August 28, 2015 1:05 PM
To: histonet@lists.utsouthwestern.edu
Cc: Cassandra P. Davis
Subject: Re: [Histonet] Frozen Section WL and Billing
A few years ago we got cited for not having the fungal control be in tissue.
The citation was from a HQIP survey we participated in from CAP. We were using
a cultured fungal specimen in a cell button at that time. Since then I have
collected and shared a number of cases we have seen with fungal
Does anyone have experience setting up a report template for a breast panel on
the Aperio? We just got ours set up and haven't had training yet. The thing has
been here since November but with holidays and schedules not working it has
been a trial. Are trying to get a jumpstart before the pathol
We have the Peloris II and VIP processors, as well as Ventana.We are happy with
the Peloris. It processes fatty tissue very well. The versatility with 2
retorts in one machine has allowed us to have several different processing
times based on tissue size. We did have a learning curve with the Pe
I agree with you in that CAP is just looking for things to change and doesn't
seem to be considering the change and decrease in staffing seen in clinical
settings. Cryostat validation? Reallycut a slide after you have cleaned and
pm'd the thing and go on. Good grief...I don't need any more p
I am wondering if anyone has hepatoblastomaeither in liver or metastatic
tumorin their tissue bank. I am working up Hep Par 1 and have a few cases
but would like a few more. We are working up this antibody to use in metastatic
case with unknown primary as a way to rule out possibilities.
Hazel,
Thanks for sharing the information about your CAP experience. With very few
outside learning opportunities at our facility I always thought CAP inspections
were a way of sharing information on tests, processes, etc. When I have been
inspecting I feel rushed to get thru the packet and tho
If you use anything higher than 70% alcohol after the 10% buffered formalin you
will have salt precipitate out and cause all kinds of problems. We use 70%,
80%, 90% alcohol in the stations after the formalin and have not seen any
issues with our Peloris or the VIPs.
Cindi Robinson HT(ASCP)
Me
What kind of slide preparation are you using for urine? Cytospin vs thin prep
vial is the discussion we are having. And are you doing a manual or automated
iron stain? Thanks for any info you can provide.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
D
I am in need of pneumocystis control. Does anyone have any to share? I have a
supply of fungus controls I can trade.
Thanks..Cindi
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com
All,
Does anyone have AFB control in human tissue they would be willing to share? I
shared fungal controls awhile back with anyone who emailed methat is, until
I ran out. I would be willing to share control material if I have something you
need.
Thanks.
Cindi Robinson HT(ASCP)
Mercy Medi
I worked as a generalist for 4 yrs and then was in Microbiology for 8 yrs prior
to moving to Histology in 1990. I have always felt the experience of working in
the other areas of lab helped me understand the entire process, especially
those involving fluids which were shared between multiple dis
238 bed acute care hospital with affiliated reference laboratory in the MidWest
is currently looking for a histotechnician or histotechnologist (ASCP
registered or equivalent preferred). Responsibilities will include, but are
not limited to, specimen processing of surgical and cytology specimen
We use safranin (used in Microbiology as a counterstain) on our small
biopsies. We apply a small drop during grossing. It does not affect staining of
H&E, IHC or ISH. We like this because it is an intense red that doesn't leach
out in the alcohols of processor.
Cindi Robinson HT(ASCP)
Mercy
All,
We have a lung case that is loaded with fungus. Is anyone in need?
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robin...@mercyhealth.com
___
Histonet mailing l
I would also be interested if anyone is using this method and their results. I
have printed off the article and shared the information with our supervising
pathologist. I have found dermal needle rollers on line but wonder if the
maximum 2 mm length will do the job of perforating the fat. Also w
Amber,
Optimizing the antibody is the first step of validation. Running the new
protocol against previously stained cases is the second step and shows you are
getting results as good as, if not better, with the new vs the old.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laborat
It is a good thing this vendor does not work in a histo lab because with that
comment/attitude he/she would not last long. I have heard the water bath theory
but upon investigation this has never been the source because every histotech I
work with cleans it each and every time. We have traced fl
Bill,
I am about the same age as you are and would like the same type of locale.
Since there are so many of us in this field due to retire in the next 15-18
years maybe we should consider investing in a Histotech Retirement Community.
Specimens could be shipped to us for processing and staining
We have oncologists ordering Her2neu on gastric cases since Herceptin has now
been approved for this treatment. Is anyone using Ventana Pathway Her2neu
antibody clone for gastric tumor? How did you do validation and what disclaimer
are you using?
Thanks for your help.
Cindi Robinson HT(ASCP
We have Cerner Millennium and have been successful having nursing order in
PowerChart and the order comes over into Pathnet. Some of the information
required doesn't transmit currently. However, it is available to the path in
PowerChart. You can contact me off line if you like, Jan. Also, I will
I do this but it has to be separate runs or wait until the kit is just running
out. I also keep the slides from the initial QC run and compare it with the new
lot to assure same intensity staining is obtained when changing lots.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laborato
We print a worksheet for each pathologist who will be reading slides
from the IHC run. If we had to circulate one sheet to get every line
signed it would be problematic as we service two locations. We just
were CAP inspected and it went great.
Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes
We are currently using 10% formalin fixation on our bone marrow cores. We fix
for 2 hours minimum prior to decal. We are using Immunocal from Decal Corp.
for 2-4 hrs followed with processing overnight in VIP. Cores are still crunchy
upon sectioning and we are doing surface decal for up to 30
We keep track of our positive cases and only cut one ribbon for use as a
positive control. This way we don't use up the block.
Cindi
>>> Rene J Buesa <[EMAIL PROTECTED]> 09/18/2008 10:51 AM >>>
The reason why the pathologists are usually reluctant to use (+) cases,
is because if they are used a
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