[Histonet] compression problems

Hi all, Has anyone experienced a problem with Paraplast Xtra over the last 3-4 months? We are going crazy with compression problems and have tried all the usual solutions (tightening all parts of microtome, changing angles, changing blades, servicing microtome) and the problem persists. This

[Histonet] human samples in research lab

Hi all, I would like to get some advice from the experts. We are a research lab currently working primarily with mouse and zebrafish tissues. One of our researchers has access to tissues from local hospitals which have been processed and embedded. Having been in research and away from the

[Histonet] oops

Hi all, Bad day here. Left the IHC stainer to attend a meeting and the volume of the DAB was not sufficient to stain all slides. By the time I realized this, they had been counterstained. I immediately applied DAB to the affected slides but naturally the antigen is not abundant if present

[Histonet] troubleshooting Oil Red O

Hi all, I'm using the Churukian method posted by Gayle Callis a few years ago and I have encountered a strange problem. The lipid stains fine, and the Gill's hematoxylin looks good before I coverslip with Vectashield Hard Set aqueous mounting medium. But within a matter of minutes, the