We use Sub-X Mounting Medium from Leica. This is what the pathologist wants us
to use.
-Original Message-
From: Allan Wang via Histonet [mailto:histonet@lists.utsouthwestern.edu]
Sent: Monday, November 13, 2017 5:47 PM
To: warda hassan
Cc: histonet@lists.utsouthwestern.edu
Subject: Re:
We are using large metal molds to embed mouse brains. We are having a hard
time getting to block out of the molds, the paraffin blocks are sticking.
Sometimes they are coming out cracked. Sometimes the cassette comes off the
paraffin block. Any idea why this is happening? Any advice on how
I am getting ready to learn how to do the LFB-H staining on brain tissue. I
was wondering want I would use for a control with this staining.
Diane
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Does anyone have a protocol/SOP for doing H LFB staining they would be
willing to share with me?
Diane L. Satterfield, BS
Manager Brain Tumor BioRepository
Research Program Leader
Duke University Medical Center
Brain Tumor Center Biorepository and Database
For those of you that use Eosin in the processing of tissue. Can you please
tell me what kind of Eosin mixture you use and how much?
Thanks
Diane
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I am in the process of setting up a Leica TP1020 processor. I was looking at
someone else's SOP where in a 100% EtOH steps they add alcoholic eosin-phloxine.
Can someone explain to me what this does and do I have to add this to one of
the 100% EtOH steps in the processing.
Thanks
Diane
I have acquired a Leica TP1020 tissue processor for my lab. I have never had
to process tissue before so I am reaching out for help. I have no idea what
protocol I need to be using. I am working with human brain tumor tissue that
has been fixed in 10%NBF for 24-48 hours then transferred to
