I have a question Lac Z staining and BetaGalactosidase IHC. The Lac-Z stain is
enzyme dependent, so it must be fresh/frozen for the reaction to work. Is
this also true for the betagalactosidase IHC? The target of the antibody is
the enzyme, but I am not sure if the enzyme need to be active
I know the potential for damage to your health is huge in histology, but are
there any studies out there that indicate histotechs are less healthy than the
average person?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Has anyone out there tried the Tissue Tek Autosection? I would love to get
some feedback on it.
Thanks.
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Hi all,
Does anyone know of a supplier that carries Finetec Cryofilm? I can't seem to
find anything. If anyone has used this, can you tell me how it compares to
CryoJane tape?
Thank you,
Liz
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is intended solely for
We are preparing paraffin slides for DNA extraction, and I am not sure if once
cut they should be kept cold before the extraction is done. We are not doing
the extraction, just the prep. If anyone else is doing this, I would greatly
appreciate some input.
Thank you,
Elizabeth
The information
Does anyone have any experience with cutting cells on PTFE
(polytetrafluoroethylene) membrane? Are there any issues cutting it with
standard FFPE methods?
Thanks in advance,
Liz
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is intended solely for the
Forgot to mention this is on FFPE tissue
From: Elizabeth Cameron
Sent: Thursday, April 11, 2013 11:21 AM
To: histonet@lists.utsouthwestern.edu
Subject: Anti-human nuclear antibody
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody
MAB1281, and if so, what
Just wondering if anyone has had any luck with Millipore anti-nuclear antibody
MAB1281, and if so, what was your protocol? We are trying to detect human
cells in mice.
Thank you.
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
The
Hello Histonetters,
I am once again requesting that you take some time out of your day to help our
facility (and yours!) with a benchmarking survey. It only takes a few minutes,
and the results will be (anonymously) shared with all survey participants. If
you work in research, a hospital,
Histonet Users,
Scientific Services at The Jackson Laboratory is conducting benchmarking
surveys in an effort to develop an understanding of the best current practices
offered by peer institutions. The goal is to gather quality data that can be
used to assist us and all survey participants
We work with mice and do a variety of fixatives, but we prefer a
methanol/acetic acid fixative. It holds the shape of the eye very well,
although it may not show the Schlemm's canal as well as other fixatives.
-Liz
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Anyone else having issues with histosearch today
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is intended solely for the addressee(s). If you believe you received this email
by mistake, please notify the sender by return email as soon as possible.
knowledgeable histotechs when these strange issues arise!
-Liz
-Original Message-
From: Cristi Rigazio [mailto:cls71...@gmail.com]
Sent: Tuesday, November 27, 2012 4:01 PM
To: Rene J Buesa
Cc: Elizabeth Cameron; histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Uneven Staining
As most
About a year ago, I posted about issues with staining mouse kidney sections
(see below for original post). We realized that part of the problem was the
sections drying during staining, which has been resolved, but we are still
having some issues with some slides staining well and others being
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth Cameron
[elizabeth.came...@jax.org]
Sent: Tuesday, November 27, 2012 12:35 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Uneven Staining
About a year ago, I posted about
Does anyone know where I can get the little cardboard tabs that you use as
placeholders when a block or slide is pulled from the file?
Thanks!
-Liz
Elizabeth M. Cameron, HT, QIHC (ASCP)
Histology Supervisor
The Jackson Laboratory
Bar Harbor, Maine
207-288-6326
The information in this email,
Thank you to all who replied!
Lab Storage Systems also has them.
-Original Message-
From: Kaye Ryan [mailto:kr...@nfderm.com]
Sent: Wednesday, October 31, 2012 10:15 AM
To: Elizabeth Cameron
Subject: RE: [Histonet] Block/Slide placeholders
Mercedes Medical sells them
Kaye
-Original
Anyone out there know of a caspase 8 that works well on mouse tissue?
Thanks!
-Liz
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is intended solely for the addressee(s). If you believe you received this email
by mistake, please notify the sender by return email
Does anyone know of a CD20 antibody that works well on paraffin embedded mouse
tissue?
Thanks!
-Liz
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is intended solely for the addressee(s). If you believe you received this email
by mistake, please notify the
Hi,
I was wondering if anyone has any suggestions for a Jones/PAMS stain that is
not working properly. This is something we don't do often. The last time we
did it was a year and a half ago, and it seemed fine at the time.
We have tried 3 or 4 protocols, including an ammoniacal silver, and it
We are doing a Hale's colloidal iron stain (no counterstain) on serial sections
of mouse kidneys. We are staining an entire kidney at a time (about 90-150
slides), and after many successful runs, we are now finding some slides in each
batch with very uneven staining. Half of a section will
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