[Histonet] setting up for staining

2015-04-17 Thread Emily Brown
Hello! My lab doesn't really do staining (H and E, Masson Trichrome) too often (maybe once a week), but I'd really like to have a set of dishes with all of the plastic bins and what not. Unfortunately, this costs $500 for just one row. Has anyone tried the newer set-ups like this https://us.vwr.c

[Histonet] Masson trichrome and H and E

2015-03-03 Thread Emily Brown
Hello! I am doing Masson trichrome manually (not with a machine) and I just found out the kit is $800!! I was thinking of buying the ingredients separately, but why the hell is it so expensive??? Also, the people I was going to borrow their reagents from said their aniline blue is not very good an

[Histonet] paraffin sectioning-dry tissue?

2015-02-05 Thread Emily Brown
Hello all! I just started sectioning mouse liver in paraffin and the tissue is very dry. I know it's not supposed to have water due to the processing, but the weird thing is that one tech's solution is to put a wet kimwipe on the block for a while. It seems to me that there is a larger processing

Re: [Histonet] Re: Embedding

2015-01-22 Thread Emily Brown
That is so cool!! I wish I had one of those!! "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Wed, Jan 21, 2015 at 1:59 PM, histot.

Re: [Histonet] anti-roll plate damage?

2014-11-08 Thread Emily Brown
Hey everyone, don't forget to "reply all" when answering questions! Is there a way to change the reply function for this list so you can reply to the list instead just the person asking a question? I don't want to miss out on any answers and I think for this question I didn't see any but the thank

Re: [Histonet] Ebola

2014-10-09 Thread Emily Brown
If formalin didn't kill CJD, what did you use? Just curious. Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives. Move forward." -Chuck Palahniuk, "Haunted" On Thu, Oct 9, 201

Re: [Histonet] Is there a Law for refusal of pathology services.

2014-08-19 Thread Emily Brown
What is the reasoning for this?? I think the insurance company would want a diagnosis! Does this person enjoy surgery for the fun of it and not actually need it? Or maybe she has another problem she doesn't want her insurance to know about? Although if she has insurance she can't be retroactively d

Re: [Histonet] HS IHC project

2014-07-21 Thread Emily Brown
I am so jealous of these kids. I didn't get near a microscope until college, let alone a microtome. I hope they have fun! Emily "By bitching and bitching and bitching, they could exhaust the drama of their own horror stories. Grow bored. Only then could they accept a new story for their lives.

[Histonet] Histology as art!

2014-06-27 Thread Emily Brown
Hello Histonetters, I'm really looking forward to going to the brand new Morbid Anatomy Museum in NYC, but imagine my surprise when I found some histology in their online gift shop!! http://morbidanatomy.bigcartel.com/category/gifts Histology is beautiful, but it is odd to look at those images on

Re: [Histonet] ISH preparation

2014-06-25 Thread Emily Brown
That may be what you have to do, as the first day of ISH needs to be RNase free! When I do them, it is 10 slides at a time and we have dishes and a lab bench set aside for RNase free procedures only. Obviously this is not feasible in all workplaces! I would just think about it logically. Use glov

Re: [Histonet] Should I leave histology world

2014-06-04 Thread Emily Brown
As someone who has been in research (basically being a histologist), I can say that there are NO jobs out there for you. The market is saturated with PhDs. Do not leave your job for a research position unless they can guarantee your salary for years. This will be very unlikely, as getting a gran

Re: [Histonet] cryosectioning, bubbles between slide and section

2014-04-29 Thread Emily Brown
Hello, This may be a little late, but we use a mix of half 30% sucrose and half OCT to section. It works really well with 14 to 25 micron sections and no cooling or heating of the slide. Our tissue is very small though--embryonic spinal cord or brain from chick or mouse. I just change the way I