Good morning out there,
We just had a request for Gallocyanine staining. Does anyone do this? Can
you share your protocol? Where do you buy your reagents?
Thanks in advance for any help in the right direction,
Eva Permaul
Georgetown University
Please, please and please help me. I am fighting a sinus infection and I
can not think to save my life.
I have to make a buffer solution:
10mM Tris-HCL, 5mM CaCl2, 50% glycerol (v/v), pH7
I have tris base and calcium chloride powder.
Can someone please help me by writing it out for me?
I am so s
abeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> l...@premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Dri
Good morning,
We recently bought MAB1281 with the hope of being able to determine if the
cells in a mouse model was human or mouse.
We want to use it to stain FFPE tissues. I have tried it with Citrate per
the companies recommendation. I ran it on a human tonsil but saw no
staining at all.
Is there
Good morning,
We used to have a ki67 antibody that detected mouse that worked really
well. It was made in rat. It is however now really weak. We have received
two different lots and it is fading fast. Could anyone recommend one that
works well for IHC-P on mouse tissues? We would prefer if it was n
Good morning Histonetters,
I was wondering if anyone has used Mount-Quick to transfer tissue from
uncharged slides to charged slides. We had a submitter give us slides for
staining on uncharged slides and the tissue comes off the slide during
antigen retrieval. I remember reading about this product
Hello,
I am looking for an HA-tag antibody to detect a protein labeled with HA in
formalin fixed paraffin embedded tissues. Could anyone suggest a published
antibody? The tissue of interest is mouse so I would prefer an antibody
made in another species.
I have found several antibodies but can't fin
We usually just use 1xTris buffered saline with 0.05% Tween.
Eva
On Tue, Apr 9, 2013 at 3:34 PM, Geoff wrote:
> You could make your own. It is usually just a buffer with some normal
> serum and Triton or Tween added.
>
> Geoff
>
>
> On 4/9/2013 2:14 PM, Perry, Margaret wrote:
>
>> I am looking
Does anyone have any suggestions?
On Wed, Oct 24, 2012 at 8:26 AM, Eva Permaul wrote:
> Good morning,
> I need to do a peptide competition for one of the antibodies we are using
> for IHC on FFPE. The company does not offer the peptide but the datasheet
> does have the sequence. Wh
your valuable advice,
Eva Permaul
Georgetown University
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-Original Message-
> From: histonet-boun...@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul
> Sent: Tuesday, September 25, 2012 1:21 PM
> To: histonet
> Subject: [Histonet] Albumin and Epcam to stain mouse cell pellets
>
> Good
Good afternoon,
Has anyone used Albumin and Epcam antibodies to stain mouse samples? If you
have would you please share the antibody information and conditions with me?
Thank you,
Eva Permaul
Georgetown University
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Histonet
? How long in
advance can I place it in the 37C before it looses its ability to be used
as an antigen retrieval?
Thanks for all your help,
Eva Permaul
HTSR
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Hi Liz,
We just used CD20 cat.no. 14-0201 from eBioscience on mouse livers and
spleen. It worked great. Email me separate if you would like to send you
the protocol.
Eva
Eva Permaul
Georgetown University
On Wed, Aug 22, 2012 at 2:48 PM, Elizabeth Cameron <
elizabeth.came...@jax.org>
I just found a nice publication with the Rm-9106 on mouse intestine. Have
provided it to my manager. Thank you everyone.
Eva
On Wed, Aug 8, 2012 at 11:38 AM, Eva Permaul wrote:
> Just to clarify. Are you using it on mouse tissues? I am only asking
> because the Thermo Scientific/Lab
I have also tried a different
antibody from Leica but got staining at the top of the crypts not at the
bottom.
Thank you to everyone for all of your valuable suggestions and advice,
Eva Permaul
Georgetown University
On Tue, Aug 7, 2012 at 2:21 AM, Bruijntjes, J.P. (Joost) <
joost.brui
@lists.utsouthwestern.edu [mailto:
> histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eva Permaul
> Sent: Monday, August 06, 2012 1:44 PM
> To: histonet
> Subject: [Histonet] Ki67 antibody to stain Mouse tissue
>
> Hello,
> I am looking for a Ki67 to stain mouse tissu
Hello,
I am looking for a Ki67 to stain mouse tissues. We have been using Dako
M2749 but just found out it has been discontinued. Could anyone suggest a
good alternative?
Thank you all for any help,
Eva Permaul
Georgetown University
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ager & Senior Scientist
> >> Tel: 612 9845 3306
> >> Fax: 612 9845 3318
> >> the children's hospital at westmead
> >> Cnr Hawkesbury Road and Hainsworth Street, Westmead Locked Bag 4001,
> >> Westmead NSW 2145, AUSTRALIA
> >
gt;
> On Jul 24, 2012, at 8:13 AM, Eva Permaul wrote:
>
> > I understand the point about the biotin and I should have said that when
> > using the ABC method we have taken to always using an avidin/biotin
> > blocking kit. We are using biotinylated secondary antibodies from
>
Hi Sarah,
I had a similar situation. If you go into your profile on the Histonet site
you can click to not receive any of the Histonet messages while you are
away. Then when you get back and want the messages again you just change it
back.
Eva
On Tue, Jul 24, 2012 at 2:35 PM, Sarah Dysart wrote:
We have used Neomarkers/Labvision cat.no. RM-9104 on mouse salivary glands
and mammary glands.
On Tue, Jul 24, 2012 at 11:16 AM, Suresch, Donna L. wrote:
> Hello Histonetters,
> Has anyone done IHC using Cyclin D1 antibody? What vendor was used? What
> control tissue was used?
> Thank you.
> D
__
> From: histonet-boun...@lists.utsouthwestern.edu [
> histonet-boun...@lists.utsouthwestern.edu] on behalf of Eva Permaul [
> e...@georgetown.edu]
> Sent: Tuesday, July 24, 2012 8:41 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: Re: [Histo
nd Fly Fishing Bum
> Orlando, Florida
>
>
> From: histonet-boun...@lists.utsouthwestern.edu [
> histonet-boun...@lists.utsouthwestern.edu] on behalf of Eva Permaul [
> e...@georgetown.edu]
> Sent: Tuesday, July 24, 2012 8:13 AM
> To: h
just weakly positive before. Just want to understand what
it is and what effects it.
Thank you all for your ideas.
Eva Permaul
Georgetown University
On Mon, Jul 23, 2012 at 7:16 PM, Tony Henwood (SCHN) <
tony.henw...@health.nsw.gov.au> wrote:
> I should have added that this was from the
in canine lymphomas, nuclear
> CD79a is a real and frequent observation.
>
> john
> IHC Services
>
> On Monday, July 23, 2012, at 08:39 AM, Eva Permaul wrote:
>
> Hello,
>>
>> I have noticed that our biotinylated secondary antibodies on occasion
>> cause
>
Hello,
I have noticed that our biotinylated secondary antibodies on occasion cause
nuclear staining in some samples. Why is this? It is not every time so I
find it rather stange. Anyone know why this is happening and what I can do
to avoid it?
Thank you for any suggestion,
Eva Permaul
Georgetown
of rabbit made PLAP antibodies from companies but the investigator wants
me to find one that has also been published.
Thank you all for your help,
Eva Permaul
Georgetown University
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Good morning,
I have been attempting to do some dual chromogenic staining. I am using
a mouse and a rabbit antibody. For the mouse antibody I am using an
HRP-mouse secondary followed by AEC for visualization. For the rabbit
antibody I am using a biotinylated rabbit secondary, followed by ABC-AP
Good morning,
I accidentally hydrated a FFPE slide that I can not stain today. It is
in water. I did not do antigen retrieval. What do I do? Can I dehydrate
the slide again? Will I be able to stain it later if I do?
Thanks,
Eva Permaul
Georgetown University
Hello,
Does anybody use an EGFR antibody for staining on mouse tissue? Is it a
published antibody? Would you be willing to share your protocol?
Thanks,
Eva
Georgetown University
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m. Is this the same
thing?
Thanks for your help of a confused tech,
Eva Permaul
Georgetown University
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these reagents
themselves. Would anyone be willing to share their recipes and how do
they compare to company bought ones?
Thanks for your help,
Eva Permaul
Georgetown University
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Good afternoon.
I just had someone ask me about staining rat tissue using an monoclonal
(mouse) antibody. I have never done this before and so was wondering if
there is anything in particular I need to keep in mind. Does mouse
antibodies cause a problem when staining rat tissues? Do I have to u
Good morning,
I was wondering if someone uses tap water to blue their slides after
Hematoxyline. If yes, do you use warm or cold water and for how long?
Thanks,
Eva
Georgetown University
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blue
hematoxylin anyways?
Thank you for all your answers
Eva Permaul
(still learning)
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Good morning out there in histoland,
I am looking for some help in finding two antibodies:
- sulfated gastrin-17 (G-7)
- sulfated cholecystokinin-8 (CCK-8)
All the antibodies that I are finding are non-sulfated.
Thank you all for your help,
Eva Permaul
Georgetown University
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