[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] (no subject)

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[Histonet] Histology Stories

I am really enjoying reading these "start-up" stories. :o) If you don't mind indulging me, I'll share mine too! I had just graduated from college with a BS in Biology when I landed a job as a cancer research assistant. Well, it turns out that portion of lab just wasn't for me! I left that

[Histonet] Microwave Tissue Processor Protocols

I am working on the validation of our microwave processors and have noticed that the tissues are a bit dry. Would anyone be willing to share their protocol timings for their fixation, ethanol, isopropyl and wax impregnation steps? We are using the Histos-5 microwaves from Milestone and are ut

[Histonet] (no subject)

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[Histonet] (no subject)

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RE: [Histonet] H&E stainer/coverslippers

We are using the Leica Autostainer XL, bridge and coverslipper (CV5030). I think they're great! Michelle _ Hotmail: Trusted email with Microsoft’s powerful SPAM protection. http://clk.atdm

[Histonet] RE: B5 fixative

Amy, We use the B+ fixative available from BBC Biochemical ( http://www.bbcus.com/products.html?pc=19&pid=57 ) My pathologists love it! Michelle > Message: 3 > Date: Tue, 02 Mar 2010 14:12:04 -0500 > From: "Amy Farnan" > Subject: [Histonet] bone marrow fixative > To: > Message-ID: <4b8d1

[Histonet] Workflow suggestions for MW processing

Thanks to all who have taken the time to reply to my question on microwave tissue processing and the resulting effect (or not) on IHC staining. As you have probably guessed, I am in the process of getting our MWs validated and set up for use. We are still using our conventional processors, but

[Histonet] Microwave processing and IHCs

Does anyone here have any experience with using microwave tissue processing and then performing IHC stains on those same tissues? We have new microwave processors and are trying to determine if we will need to get all new controls for our IHCs that have been microwave processed. If yes, we wi

[Histonet] (no subject)

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[Histonet] Microwave Tissue Processing

We are getting a microwave tissue processor and the question has come up as to whether or not a lab aide is allowed, per Florida requirements, to run the processors. These processors are semi-automatic and require involvement by staff to go from one step to the next. Does anyone have any expe

[Histonet] flammable liquid storage

I was told that we are allowed to have 2 gallons of flammable liquid contained within a flammable cabinet per 100 sq. ft. My question is the following: Is that square footage for the *entire* clinical laboratory or *only* the histology area of the lab? My inclination is to say the entire cli

[Histonet] RE: Eosin and Leica XL stainer

Cheryl, Yes, the Lecia XL is supposed to vibrate when it lifts the racks. If it's not doing that, you need to call and ask for some assistance. It sounds to me like there is something either turned off or broken in the setup. Regarding the eosin getting the alcohol pink, that happens to us a

[Histonet] Downtime Procedure

Does anyone have specific downtime procedures for when the Anatomic Pathology area computer goes down? The AP area in my facility does not have a downtime procedure and we ran into an issue yesterday when the computer system went down. We processed, on the fly, as it were, but I now recognize