[Histonet] Coverslip Sealant

Hi there, Does anybody know of a good alternative to nail polish for sealing round coverslips (or any shape, for that matter!). Ideally I am looking for a solution that is both an aqueous mount media and sealant, if such a beast exists... Thanks! Gil PS: Happy New Year! -- Guillermo Palchik

[Histonet] good protocol for ICC in neurons

a good working protocol that could share with me? It would be greatly appreciated... Thanks Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical Center Research Building Room W 217 3970 Reservoir Rd. NW, Washington, DC 20007 Lab: 202-687

[Histonet] TUNEL of frozen thick rat brain slices.

, could I do overnight incubations? the tissue is flash frozen, but it does get fixed at the beginning of the protocol with PFA (4%) and subsequently with acetic acid-EtOH. Thanks, Gil -- Guillermo Palchik Ph.D. Candidate - Interdisciplinary Program in Neuroscience Georgetown University Medical

[Histonet] Issues regarding flash freezing and cutting of Rat pup brains

Dear Histoneters, I am looking for help regarding flash freezing of rat pup brains (Postnatal day 8). We need the tissue to be fresh (unfixed) for cutting at the cryostat. So far the technique has been: 1- to scoop the brains straight into cold (-50 C) Isopentane for about 10 seconds,

[Histonet] Fixation question - Cerebellar granular cells

about the sucrose? does it remove the water? I'd appreciate your thoughts... Best, Guillermo Guillermo Palchik g...@georgetown.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] curling on cryostat brain sections and other issues

Hi there, I am having problems with my brain sections (15 um - flash-frozen) curling up once I remove the antiroll plate on my cryostat... does anyone know how i can prevent this? Also - does anybody have a quick guide on what to improve while cutting, based on the damage seen on the

[Histonet] TUNEL on fixed brain tissue

Dear Histologists, Does anyone have a good protocol for doing TUNEL on fixed brain slides? Thanks Guillermo Palchik g...@georgetown.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo

[Histonet] TUNEL on FIXED rat PUPS

as a perfusate and see if this would allow us to do the TUNEL. I would appreciate any comments and suggestions regarding this. I am sorry for the lengthy email, but I wanted to show a more or less complete picture, in case I am overlooking other factors… Guillermo Palchik g...@georgetown.edu