Ever tried turning the handle of the cryostat, when it's at room
temperature?
Cryostats are tooled manufactured to operate at a low temperature.
Since metal contracts at the low temperature, you'll find that you can't
operate the microtome at the higher temperature. The handle will barely
move.
Dear Merissa,
I have used the Labconco countertop fume hood in a few different
Histology settings, both MOHS and general, and found it to be very
compact, easy to change the filter, and very effective at pulling the
fumes away from the user. It is also a ductless system.
See
Could I get some feedback on linear stainers?Has anyone had any
experience with the Leica ST4020?
We are going to purchase a linear stainer for a new MOHS laboratory.
Thanks,
Sandy Harrison
Histology Supervisor
VA Health Care Systems, Minneapolis
612-467-2449
Does anyone have a procedure for restaining a cytology slide? We
experienced a loss of cells on an FNA aspirate of a lymph node, which
had been originally stained with Diff Qwik. The procedure we used was
as follows:
1) Destain using 1% Acid Alcohol (1 ml. concentrated HCL added to
99
We do an H.P. routinely on stomach, body, fundus, antrum, pyloric,
prepyloric, and gastric (Not on cardia area of stomach or GE jcn.)
We use a kit from Newcomer Supply of a Steiner-Chapman modified Silver
Stain.
The pathologist's order an IHC for H.P. only about 5% of the time, if
for example,
I just purchased 6 Slide Mates. We just began using them yesterday.
Are any of you using slides that are NOT Fisher slides on your
SlideMates?
When you respond, could you answer the following questions?
a) Is the print job consistently satisfactory?
b) How long have had
We have 2 Bond Max instruments and have experienced multiple heaters
getting contaminated and corroded underneath, with a particulate matter,
causing the heaters to fail. Our 2nd BOND Max is 3 years old and we
have had to replace a total of 12 heaters (out of 30.)
The only thing we do that is
Leica Autostainer XL with CV5030 coverslipper and transfer station.
This has been a real timesaver for us. It automatically moves the
slides from the stainer to the coverslipper. It has been relatively
trouble free.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Depends ;-)
All kidding aside, there are factors that weigh in, such as geography,
availability, training, experience, speed, etc.
10 years ago, when I worked as a Mohs' tech in the Denver, Colorado
area, I received $25.00 per hour, plus benefits. I usually only worked
3 days per week, but on
Yes, we have a wireless temperature monitoring system, called Checkpoint, and
each room has a sensor that monitors temp and humidity.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
And what about a paperless procedure manual?
We have an electronic system, called Proquis, for our manuals. It sends
an e-mail to all the appropriate people, when there is a new procedure
to review or when old procedures are due for review. Pretty cool.
The only drawback is, most of the
1) Does anyone have a Leica Cassette Printer on which they are
printing 2-Dimensional barcodes?
2) Is anyone using a Thermo slide printer, with bar code reader, to
read cassettes printed off of a Leica Cassette Printer with 2-D bar
codes?
Thanks for your input.
Sandy C.
Does anyone have ordering information for a stain called Chicago Sky
Blue(CSB)? I just saw an article in the NOV issue of ASCP LABMEDICINE
(page 649) comparing KOH vs. CSB and, wow, is that a beautiful stain.
The results were dramatic, with about a 25-50% increase in the
Dermatophytes
Does anyone know if it is standard to save sections from between the
levels for IP? Is it standard at your institution?
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
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I have been told that I can no longer buy only the replacement ink
cartridges for Leica cassette and slide printers. The ink cartridge was
already an outrageous $411.00 each. Now you have to by a kit for
$566.00 each. The kit includes a replacement plate along with the ink.
Why in the
Could someone share their gout processing procedure? The specimen is
fresh, not in formalin.
Thanks,
Sandy C. Harrison, HTL (ASCP)
Histology Supervisor
Minneapolis VA
612-467-2449
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Histonet mailing list
Hi Dorothy,
Try Richard Allan Bluing Reagent.
Here's what they say about their product: It is a buffered product
that ensures the proper alkalinity (pH=8.0). Unlike ammonia and lithium
carbonate, RA's Bluing Reagent does not allow for the pH shift which can
affect the crispness of nuclear
We do either IHC or a Modified Steiner, depending on the Pathologist's
preference. We switched to Newcomer Supply's Steiner-Chapman Modified
Silver Stain Kit about a year ago, because it eliminated the use of
Uranyl Nitrate. We have been delighted with the consistency of the
stain.
Sandy
Out of 6 recent hires, I hired two 50+ employees. One had been employed
in another department for over 28 years. One was a fairly recent grad.
of a Histology program. They were both good employees, though neither
had much routine Histology experience.
That being said, neither one of them are
We dry the control slide at the same time that we dry the tissue being
stained, since controls are supposed to be handled in the same manner as
the test tissue. That being said, I guess if we were truly following
that rule, we would cut the control on the same day we cut the test
sample. :-)
Wow...didn't realize how addicted to Histonet I'd become till
this week!
Sandy Harrison
Histology Supervisor
VA Medical Center
Minneapolis, MN 55417
612-467-2449
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu]
CBG Biotech. Their contact info is 1-800-941-9484 or you can e mail
them at i...@cbgbiotech.com. I purchased my recycler through them and
have ordered extra hydrometers from them, too. They give excellent
customer service.
Sandy Harrison, HTL(ASCP)
Histology Supervisor, VA Minneapolis
I'm looking at the Slide Mate and Print Mate, too. Currently, I have
the Leica cassette and slide printers. They have really been great.
Reliable and relatively problem free. But they are so large. I'd like
to find a slide printer that has a small footprint, like the Slide Mate,
so that we can
Here's what the recently revised CAP checklist says about releasing blocks;
scroll down to Note 2:
**REVISED** 06/17/2010
ANP.12500 Record Retention Phase II
Surgical pathology records and materials are retained for an appropriate period.
NOTE 1: Minimum requirements for surgical pathology,
We have the Leica Cassette Labeler. It works just fine. I have looked
at the Thermo Fisher Cassette Printer, which had a much smaller
footprint than the Leica. I would be tempted to go with the Thermo if I
have to replace my current labeler.
These are expensive instruments but worth having.
About 1/2 of my tech.'s wipe the oil off; otherwise it causes the first
2-3 ribbons to curl off the knife, rather than forming a nice ribbon.
Sandy Harrison
Histology Supervisor
Minneapolis VA
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
You have included all the criteria; fixation, processing, embedding,
microtomy, staining, coverslipping and labeling. If you were able to
submit slides to CAP under their HQIP program, you would get graded
evaluations. Short of that, could you perhaps send out duplicate slides
to another local
Dear Histonetters,
If you use a recycler for xylene and/or alcohol, how are you labeling
the recycled containers? Do you assign a lot # to each carboy?
Do you keep a log or just label any container filled from a carboy with
the lot # and the %, in the case of alcohols?
Thanks,
Sandy
If there are no expiration dates on the chemical:
For chemicals distributed by Fisher, look at the Lot #. The first 2
numbers are the year it was manufactured. In general, chemicals are
considered expired 3-5 years after the year they were manufactured.
You can also check the certificate that
DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN.
Full time Histotech. opportunity at Minneapolis VA.
BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp.
IHC experience a plus. Effective interpersonal skills required.
Holiday, evenings and weekends off. Excellent
DEPT. OF VETERAN AFFAIRS MEDICAL CENTER, MINNEPOLIS, MN.
Full time Histotech. opportunity at Minneapolis VA.
BS or BA in Biology. HT cert. required, HTL preferred. Prefer 5 yr. exp.
IHC experience a plus. Effective interpersonal skills required.
Holiday, evenings and weekends off. Excellent
How about microwaving to dry slides? Can that cause nuclear bubbling?
When you say completely drained off, does that mean your slides have to be
completely dry prior to placing them in the oven?
Thanks,
Sandy
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
polycyclic aromatic flourescent compounds that in high concentrations
I wouldn't think the 3 mls of eosin dropped in the last 95%
alcohol could be considered high concentration but that's what keeps
Histonet entertaining; I learn something new every day.
-Original Message-
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