I would suspect osmium would destroy the fluorescent proteins the PI wants to
detect, while staining fat droplets nicely. You can always ask your friendly
EM lab to do the staining to find out, they have to use it and are familiar
with the proper ways to deal with it.
We have used handheld digital cameras for our research in sheep in Galveston.
For autopsies at the Shriners hospital, we use handheld cameras, one operated
by a professional photographer, and also an old copy stand with hot lights and
a backlight and a Sony digital camera with a macro lens on
Way back when I was at Emory, the GI docs used to place their biopsies on
freshly cut slices of cucumber. The cucumber slices were fixed and processed
with the specimen, cut nicely, and support the tissue in a perfectly flat
orientation. I discourage use of the standard blue gauze pads
We briefly experimented with using a fixative for stillborn fetal brains that
was a mixture of equal volumes of 20% formalin and 95% ethanol. The gross
preservation and firmness of these very soft brains was improved, and the
histology results were fine, but as one might predict, some
We do this routinely at the Shriners Burns Hospital in Galveston when asked to
look for fungi in infected burn wounds and provide a "rapid section" report.
It works very well when the timing is carefully controlled by frequent visual
inspection. We use a paraffin section as a control. We
The old Sorvall JB-4 microtome was designed for this purpose and works well.
They are available at a reasonable price from several used equipment providers
and on Ebay.
From: Goetz, Freya E. via Histonet [histonet@lists.utsouthwestern.edu]
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