[Histonet] Spirochete Sensitizer

2011-09-12 Thread Hayes, Randi (HorizonNB)
Hi everyone, I'm wondering what people are using as a Spirochete Sensitizer. It's been suggested to us to use a 1% Uranyl Nitrate solution but we're a little wary of making our own solution (nasty stuff!). Does anyone know if someone sells this commercially, not in a kit? If not, is there

[Histonet] Procedure for making Gram Control

2011-03-16 Thread Hayes, Randi (HorizonNB)
Hello out there in Histoland, I'm looking for a procedure for making your own Gram controls. Any assistance would be appreciated. Thanks, Randi /pre--- Horizon Health Network Disclaimer ---brbrThis e-mail communication (including any or all attachments) is intendedbronly for the use

[Histonet] IHC Reference Resources

2011-01-31 Thread Hayes, Randi (HorizonNB)
Hi to everyone in Histoland, I'm working on beefing up my resource library for IHC. What are some publications that you just couldn't go without? Which provide the most complete antibody information that is on the market today? Thanks for your input! Randi Hayes Histology Supervisor, The

[Histonet] Manual Coverslipping Safety Issues

2011-01-04 Thread Hayes, Randi (HorizonNB)
We haven't manually coverslipped here in almost 5 years. The cost of the coverslipper as well as the consumables is still cheaper that a lab assistant (at a full pro-rated wage including pension benefits). We've also been able to extend it's usefulness to other departments in our laboratory who

[Histonet] Re: positive slides

2010-12-07 Thread Hayes, Randi (HorizonNB)
The VWR slides (superfrost catalogue #48311-703) are the only slide that is validated to be used on the Ventana platforms. The problem with these slides is that they are hydrophobic. They do hold onto the tissue really well, but when you dip them in a water bath or pipette fluid on them while

[Histonet] Using GEWF solution and IHC staining

2010-07-27 Thread Hayes, Randi (HorizonNB)
At a recent conference, our PA learned of using GEWF (glacial acetic acid, ethanol, distilled water, 40% formaldehyde) solution as an aid for Lymph Node retrieval in Colorectal Cancer resections. Although a good idea, I'm wondering how safe it is to use when staining for IHC. Does anyone have