[Histonet] FISH signals

Hi, how the FISH signals in FFPE tissue can be strenghten? by standard procedure only weak signals for EGFR can be obtained, so far I tried different time in Vysis protease 1 (45, 60, 70 min), for other probes 70 min works quite well on our FFPE, but not for EGFR? what other modifications can

[Histonet] cryostat

Hi,I wonder what is the way of removing shavings/trimmings from the cryostat in other lab?, with the wet paper? gauze?, household vacuum cleaner - yes I saw this in one lab!?thanksIrena ___ Histonet mailing l

RE: [Histonet] Urine cytologies

we prefer filtration of urines through 5 mikron policarbonate filters Irena Kirbis Ljubljana, Slovenia > To: bryan.wat...@neiurology.com > From: dkb...@chs.net > Date: Tue, 25 Oct 2011 09:46:06 -0400 > Subject: Re: [Histonet] Urine cytologies > CC: histonet@lists.utsouthwestern.edu; > histonet-

RE: [Histonet] Paraffin blocks from cell line

we made a cell block from cell line by following procedure: - centrifuge cell suspension - fix the sediment overnight in formalin (for tissue fixation) - optional, the button will be anyway fixed by subsequent procedure - centrifuge and decant the formalin - add few drops of liquid agar (not too

RE: [Histonet] ArrayMold System

Hi, we have Arraymold more than a year now and we prepared around 10 TM's with it, it is really simple, easy and effective, however we found instructions to keep contructed TM overnight on 37 degrees uneffective since recipient and donor paraffin were not blend enough, we keep our TM's overnigh

[Histonet] infectious fixed tissue and cells

Dear all, could anyone out there provide a scientific reason for wearing gloves handling slides with FFPE sections and FIXED cytology slides? I could understand that by wearing gloves we could prevent epithelial contamination of cytology slides but I would like to know which microorganism could

[Histonet] fixed cytology slides

Greetings to all, so far we handled wet-fixed cytology slides as health risk free without gloves, however there is no data regarding this on the net (or at least I wasn't able to find it). I would appreciate response on safety policy in other cytology labs regarding fixed cytology slides and/or

RE: [Histonet] Thyroid Smears

I guess that you're staining these fix slides by Papanicolaou?, perhaps the smears are too thick?, we process daily different kind of smears and always prepare one fix and one air dry smear for Pap and Giemsa staining and occasionaly we have an issue only very muccous samples which can coming

RE: [Histonet] ArrayMold System

so far we have good experiencies with it, it's very easy to use eg. it's easy to prepare and construct TM, however we still struggle how to handle prepared TM in order to keep all punches on the cut sections, it seems that this part request some additional adjustment - at what temperature and h

RE: [Histonet] RE: Edge affect on TMA?

how to prevent spots coming off during sectioning TM's? we do sometimes facing problems sectioning TM's, they're just falling apart? thanks for any hints Irena > From: hfe...@jhmi.edu > To: sette...@umdnj.edu; ahut...@dh.org; histonet@lists.utsouthwestern.edu > Date: Tue, 21 Dec 2010 16:13:31 -

RE: [Histonet] carnoys fluid

I would recommend methanol or ethanol for fixation, smears itself can be culprit of inconsistent staining Irena > To: histonet@lists.utsouthwestern.edu > From: heather.mcl...@webmail.co.za > Date: Wed, 24 Nov 2010 19:42:51 +0200 > Subject: [Histonet] carnoys fluid > > Dear Histonetters, > > We

RE: [Histonet] RE: Saponin Technique

for removing erythrocytes from hemorrhagic cytology samples we use filtration of bloody suspension through membrane filter with pore size 20 mikrons, you can find an article in Cytopathology. 2007 Jun;18(3):175-9. Epub 2007 Mar 27. it is worth trying! regards Irena > From: tmcne...@lmhealth.o

RE: [Histonet] Urine Cytology

there are different slide preparation techiques used in cytology labs - ThinPrep methodology, cytocentrifugation, membrane filtration are the most often used, technique depends on pathologists preferencies and equipment and experiencies of the lab! hope this helps Irena > From: sjkit...@live.

RE: [Histonet] Re: How to troubleshoot a cytology slide with dehydration problems

Dear Valerie, I can only agree that Papanicolaou stain is very tricky, having 20 yrs experiencies with it! looks very simple but you need to know some basic rules. first of all the slides should be hydrated at the beginning of staining procedure since the hematoxylin is water based stain! it se