[Histonet] Paraffin for Infiltration and Embedding Validation

2014-09-11 Thread Ian R Bernard
respond the more references I have to make an informed decision as to choice and eventually quality. V/r Ian R. Bernard, MSHA, HT (ASCP) HTL (Pend-2014) USAF, MSgt (Retired) Air Force Red Cross Volunteer Histologist (since 1 June) Anatomic Patology Technical Supervisor (effective 22 Sep) Anatomic

[Histonet] RE: processing biopsies on VIP

2014-08-28 Thread Ian R Bernard
Check your protocol. Heat or temp is a factor and length of time in alcohol. We have P/V for the 10 % NBF and paraffin infiltration. IB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Algeo, Lacie A

RE: [Histonet] Peggy Wenk's passing

2014-07-28 Thread Ian R Bernard
On behalf of all military histologist who knew Peggy, please accept our condolences. I had the pleasure of attending her NSH seminars and seeking out questions from her as recently as a few months ago. Sorry to hear of her passing. She was a true champion in the field of Histology and now

[Histonet] Template Special Stain validation Form or Sheet

2014-06-25 Thread Ian R Bernard
Any suggestions or examples (from CAP, ASCP or NSH) on a template validation sheet that we could use for documentation and for CAP inspection purposes? Is there a recommended test or sample size. We have positive controls for all our test menu stains. Need a reference for all above. Ian

[Histonet] RE: Special stain control validation- CAP compliance

2014-05-01 Thread Ian R Bernard
Documentation is a key aspect of lab work. It is almost like doing the practice without procedures. Its shows history, continuity and evidence that the work was methodical, approved and sound. Thus it provides evidence based substantiation. Put all you do in practice in written language and

RE: [Histonet] RE: competency form

2014-04-09 Thread Ian R Bernard
There is a great need for standardization in histotechnology, from forms, policies, procedures/methods to controls processes and protocols. Hope NSH is working with ASCP and CAP to make this happen. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic Pathology Lab Manager USAF- Active Duty 210

RE: [Histonet] RE: competency form- Inspector Perspective.

2014-04-09 Thread Ian R Bernard
, submit your evidence of compliance and the Lab Accreditation Committee will either overrule or sustain. I suspect they will overrule. Just saying, but based upon just what you described, it sounds like you all met the intent of the question. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic

[Histonet] Best Study material for HTL

2014-03-02 Thread Ian R Bernard
. Consulting, Inc. Please advise. MSgt Ian R Bernard, HT(ASCP), MSHA-UAB Anatomic Pathology Lab Manager USAF- Active Duty 210-687-7540 Cell ibern...@uab.edu ian.bern...@comcast.net ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

[Histonet] Rush Processing Protocol

2013-11-29 Thread Ian R Bernard
Fellow Histonetters, our laboratory mostly processes small biopsy specimens such as GI, skins, vas deferens, ECCs, Cervical biopsies, EMBXs and breast core bxs with our largest routine specimen being gall bladders. We are seeking recommendations on a proven same-day or less than 8-hour

[Histonet] Ideal Workflow Lab Design for Histo/Cyto lab Processes

2013-10-31 Thread Ian R Bernard
Histonetters, I'm looking for information or references concerning the ideal workflow design for a Histo/Cyto lab services. Need to consider ergonomics, work space, equipment, safety, etc. I know there are info. Just need source and experts to chime in. Journal of Histotechnology Advance

RE: [Histonet] Cytopathology Quest.

2013-10-14 Thread Ian R Bernard
Thanks for your reference and input IB -Original Message- From: Fimbres, Amber [mailto:afimb...@uci.edu] Sent: Monday, October 14, 2013 11:58 AM To: Ian R Bernard; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Cytopathology Quest. According to Koss' Diagnostic Cytology

[Histonet] Gastro Tissue for Validation of Processing Protocol

2013-09-17 Thread Ian R Bernard
Our lab is looking to acquire actual (gastric) or alternate similar tissue to validate a gastro tissue processing protocol. Any suggestions on obtaining endoscopic biopsy specimens or alternate tissue types to validate the gastric biopsy protocol? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP

[Histonet] Retirement of Mr Bryan Haynes from Civil Service as Histologist- USAFA

2013-08-18 Thread Ian R Bernard
capacities during his tenure as an USAF active duty person and as a DOD GS civilian in the histology career. Please send your quote about Mr. Haynes as a Friend, colleague, mentee or mentor, etc. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group

[Histonet] RE: Biocare Decloaker Nx Gen

2013-08-17 Thread Ian R Bernard
We recently bought this equipment and it works well. We are happy with it. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Thursday, March 28, 2013 1:52 PM To: Swartwood, Steven J;

RE: [Histonet] Telepathology

2013-06-12 Thread Ian R Bernard
We are a test site for the Aperio System. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gloria Tharp Sent: Tuesday, June 11, 2013 9:09 AM To: Histonet@lists.utsouthwestern.edu Subject: [Histonet]

RE: [Histonet] Happy Lab Week from Pam Barker and RELIA Solutions!!

2013-04-22 Thread Ian R Bernard
on another day horseback riding. That way our mission is staffed without negative impact to patient care V/r Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540 -Original

[Histonet] 2013 CAP Inspection Success National Lab

2013-04-19 Thread Ian R Bernard
Colorado Histo colleagues, I'll see you tomorrow for the Colorado Society of Histotechnology Meeting in Fort Collins. V/r Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP)- (Working on HTL) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate

[Histonet] FFPE for Immunohistochemistry.

2013-04-11 Thread Ian R Bernard
Pretreatments are used to recover bonded antigen sites owing to formalin linkage. What is the optimum or maximum fixation time for tissues that may require Immunohistochemistry staining? IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Validation Method for Auto HE stainer

2013-03-28 Thread Ian R Bernard
I'm looking for a benchmark or researched protocol. IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] RE: Biocare Decloaker Nx Gen

2013-03-28 Thread Ian R Bernard
I would welcome input hear as well since we are looking at buying one as well. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Swartwood, Steven J Sent: Thursday, March 28, 2013 12:40 PM To:

[Histonet] Optimum room temperature for Histo Cyto Lab

2013-03-27 Thread Ian R Bernard
Does anyone know about research or standards with this. Deg C or Deg F IB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Quality In AP

2013-03-27 Thread Ian R Bernard
and reduce errors for patient safety. IB -Original Message- From: Cristi Rigazio [mailto:cls71...@gmail.com] Sent: Sunday, March 24, 2013 9:58 AM To: Ian R Bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Quality In AP During a tumor board conference, a pancreatic

[Histonet] RE: Procedure manuals

2013-02-01 Thread Ian R Bernard
Pre CAP every two years or as needed. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Gail Marcella Sent: Friday, February 01, 2013 11:59 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

[Histonet] RE: cytoprep

2013-01-30 Thread Ian R Bernard
Our rehydration time is 30-45 secs. Then into 95% alcohol. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Wednesday, January 30, 2013 12:18 PM To: histonet@lists.utsouthwestern.edu

[Histonet] CSF GYN Testing

2013-01-27 Thread Ian R Bernard
What is a perfect amt/size specimen for CSF for Cytology non gyn testing per the literature? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) NCOIC-Manager, Anatomic Pathology Lab 10th Medical Group USAF Academy, CO 80840 Graduate Certificate In Gerontology Student-UAB 210-687-7540

[Histonet] CAP New requirement: Cytopathology Exclusions from Submissions

2012-12-30 Thread Ian R Bernard
Fellow Laboratorians who process Cytopathology specimens, specifically, Non-Gyn specimens. A policy that list specimens that are excluded from routine submission to cytology for processing. Please share your facilities' approved examples on the types of specimens, and a justification and

RE: [Histonet] CAP New requirement: Cytopathology Exclusions from Submissions

2012-12-30 Thread Ian R Bernard
Yes, This is a new checklist item as of 07/11/2011. Cytopathology Exclusion question: CYP.01650. Ian -Original Message- From: Oscar [mailto:omgwake...@gmail.com] Sent: Sunday, December 30, 2012 9:15 PM To: Ian R Bernard Subject: Re: [Histonet] CAP New requirement: Cytopathology

[Histonet] FNA procedure in Cytopathology

2012-12-30 Thread Ian R Bernard
With HIPPA privacy rules, patient identifies should be carefully considered. For the time out procedure for patient safety, during the FNA process ,we ask the patient to provide DOB and name only. For the slide labeling part of the process, what two patient identifiers do you all annotate on

RE: [Histonet] Re: Stain for HP

2012-10-19 Thread Ian R Bernard
Also Alcian Yellow -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bob Richmond Sent: Friday, October 19, 2012 5:01 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Stain for HP Staining

[Histonet] RE: H.Pylori

2012-10-17 Thread Ian R Bernard
We do Alcian Yellow for HP. IB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Harrison, Sandra C. Sent: Wednesday, October 17, 2012 2:04 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet]

[Histonet] RE: Used Histology Equipment

2012-10-12 Thread Ian R Bernard
IMEB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Best Sent: Friday, October 12, 2012 5:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Used Histology Equipment Does anyone have

RE: [Histonet] Are those sites using the Thermo Fisher SlideMateslide printer also using Thermo slides?

2012-10-12 Thread Ian R Bernard
Encouraging information and thanks for the slides tibit. That has been an issue. IB -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Laurie Best Sent: Friday, October 12, 2012 5:38 AM To: Beth Cox;

RE: [Histonet] Are those sites using the Thermo Fisher Slide Mate slide printer also using Thermo slides?

2012-10-10 Thread Ian R Bernard
We have also acquired a Thermo Fisher slide mate/micro writer in Mar/Apr of this year. We have yet to have them work and consistently. The printing quality despite the type slides used is inconsistent. Nevertheless, I am giving them opportunity to fix it since we have invested money. IB

[Histonet] RE: Breast processing

2012-10-08 Thread Ian R Bernard
This suggestion may not apply to reference laboratories, since these labs tend to work over the weekend. However, in our case, at an ambulatory primary care and surgical specialty clinic, I was able to work with our Ultrasound (Diagnostic Imaging) and ENT departments to have the breast core

[Histonet] RE: Negative Controls

2012-10-04 Thread Ian R Bernard
This is the key. What is the evidence based research/studies to support this? I will continue with negative controls until I am able to get peer reviewed research to support this. Besides overkill maybe pricey ($$) at times or not (since it is just slides) but from what I learned this past

RE: [Histonet] Tissue grossing

2012-09-16 Thread Ian R Bernard
Kristen hope this helps. See CAP requirement for other than a pathologist assistant or pathologist below. Our lab policy is to use the histotechnician certification HT(ASCP) or HTL (ASCP) as qualification to gross since there is an educational requirement for certification that is comparable

[Histonet] Let's Talk CAP Inspections

2012-08-28 Thread Ian R Bernard
I trust all of us will hopefully benefit from this line of discussion: In 2013 our lab will undergo our 2013 CAP inspection. The Cyto (Non-Gyn)-pathology, and Anatomic(Surgical only) Pathology and now the new Commons checklist will be used. Over the next weeks, in an effort to prepare, and

RE: [Histonet] Microtome feedback

2012-08-18 Thread Ian R Bernard
What is great about the Leica RM2255? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Bea DeBrosse-Serra Sent: Friday, August 17, 2012 8:58 PM To: Fred Underwood; Histonet Subject: RE: [Histonet]

RE: [Histonet] Immuno Controls

2012-07-04 Thread Ian R Bernard
Looking for the same Ian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Carlos Hernandez Sent: Tuesday, July 03, 2012 11:34 PM To: Histonet Subject: [Histonet] Immuno Controls Hi All! I was wondering if

[Histonet] Muscle Biopisy Procedure

2012-06-22 Thread Ian R Bernard
in Washington DC for processing. We will not perform any other aspects of the procedure, just the freeze and ship. Hope to do this next week. Any assistance is greatly appreciated. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840

RE: [Histonet] Reagent rotation

2012-06-05 Thread Ian R Bernard
We do it by number of blocks- At or after 350 blocks the processor is changed. Ian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Stephenson, Sheryl Sent: Tuesday, June 05, 2012 2:03 PM To: 'Mark

RE: [Histonet] certification of histotechnologists

2012-05-25 Thread Ian R Bernard
our professional culture and the history of our profession. It will instill major professional pride. V/r IRB Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[Histonet] Cytology in Histo Lab

2012-05-16 Thread Ian R Bernard
Our histopathology lab does cytology processing of Non-Gyns samples. Looking for a good cytology spray fixative to fix nipple discharge slides or smears. Need name of product, catalog number and distributor of this product. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group

[Histonet] Tissue Processing Protocol for Small iopsies

2012-05-13 Thread Ian R Bernard
this to try and split cases to avoid cross contamination at the grossing, embedding and cutting. We consider this a QA mechanism for maintaining the integrity of the specimen cases. V/r Ian R. Bernard Ian R. Bernard, MSgt, USAF, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab

RE: [Histonet] Can use some help with processing references

2012-05-13 Thread Ian R Bernard
does. Hope this helps. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Jackie O'Connor

[Histonet] RE: Tissue Processing Protocol for Small biopsies part 2

2012-05-13 Thread Ian R Bernard
activity. Though I'd double check myself. Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP) 10th Medical Group- Anatomic Pathology Lab USAF Academy, CO 80840 From: Ian R Bernard Sent: Sunday, May 13, 2012 11:15 AM To: 'histonet@lists.utsouthwestern.edu' Cc: BERNARD, IAN R MSgt USAF USAFA 10 MDSS/SGSH

[Histonet] New Topic: New Tissue Processor

2012-05-13 Thread Ian R Bernard
, if there are any military or Federal agencies out there with this processor, please respond. However, I will take all responses. Note: This is no Federal endorsement of the Leica or Sakura processors, just our lab's preference based upon experiences/feedback. Ian R. Bernard Ian R. Bernard

[Histonet] Tendon Tissue Specimens

2012-05-13 Thread Ian R Bernard
like the endoscopic tissues), and we do some surface decalcifying. Besides these two techniques, are there are other methods for obtaining optimum sections (I.e. full face and complete), e.g. processing protocol, soak in another reagent etc.? Ian R. Bernard Ian R. Bernard, MSHA, HT (ASCP