Re: [Histonet] IHC validations

2020-06-02 Thread Ingles Claire via Histonet
Paula: Usually CLIA follows CAP's lead or has less strict guidelines. Following the CAP guidelines should be good for both. Claire From: Paula via Histonet Sent: Tuesday, June 2, 2020 11:25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] IHC validati

Re: [Histonet] UV Light in Fume Hood

2020-05-14 Thread Ingles Claire via Histonet
UV-C wavelength is the best for the virus killing. Claire From: Paula via Histonet Sent: Thursday, May 14, 2020 1:28 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] UV Light in Fume Hood WARNING: This email appears to have originated outside of the U

Re: [Histonet] guidelines for using a microwave designated for food, but used to heat up histogel

2019-12-12 Thread Ingles Claire via Histonet
OMG!!! really?!? OSHA, Joint Commission, CAP, ANY regulatory agency. Give them the lounge microwave and get a new one for your break room immediately!! Fumes, contamination on the outside of whatever it's in, etc. Not even mentioning if it gets spilled. But then again, our docs like to store Bot

Re: [Histonet] Flammable Sprays in Cryostats

2019-09-25 Thread Ingles Claire via Histonet
Liquid Nitrogen guns. Claire From: Knutson, Deanne via Histonet Sent: Wednesday, September 25, 2019 7:50 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Flammable Sprays in Cryostats WARNING: This email appears to have originated outside of the UW

Re: [Histonet] Xylene substitutes for clearing agents

2019-08-28 Thread Ingles Claire via Histonet
Chris: Propar from Anatech works great for us. I believe it is still advisable to use xylene in the cleaning cycle on the processors though. We had to go back the other way a bit when our Doc wanted a tape coverslipper. Now he gripes about the xylene smell. Hmmm. Claire

Re: [Histonet] Softening Calf Hooves

2019-03-21 Thread Ingles Claire via Histonet
Sandra: We here at the Dermatopathology department of UW Mad have found: -10% Potassium Hydroxide works best for us on our nails. -We usually only need to have it in solution for a few hours. As you are trying to soften hoof, I might play with both concentration and time in solution. - I don't b

[Histonet] ST4020 Stainer

2019-02-15 Thread Ingles Claire via Histonet
Netters! A question on behalf of a co-worker. We are in the process of validating the ST4020 stainer from Leica. We are a Mohs clinic that currently uses Carazzi's double strength Hematoxylin. We are looking for a better staining protocol. We are planning on moving to a Harris formula when s

Re: [Histonet] Processing cystic tissue

2017-04-04 Thread Ingles Claire via Histonet
Charles: I usually get out as much of the cystic material as I can without damaging the cyst wall itself. That is all the docs are looking at anyway. I am assuming you are referring to skin cysts, not ovaries, etc. Claire From: Charles Riley via Histonet

Re: [Histonet] Reference for this Recipe and Use

2017-03-27 Thread Ingles Claire via Histonet
Sadly, it is much safer than the old Chloroform clearing method. Claire From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu] Sent: Sunday, March 26, 2017 6:22 PM To: ian bernard Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histo

Re: [Histonet] Performing PAS with Diastase by Hand

2016-10-05 Thread Ingles Claire via Histonet
We get our Diastase powder from American Mastertech. Claire Dermatopathology lab University of Wisconsin Hospital and Clinics From: Vickroy, James via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 3:48 PM To: histonet@lists

Re: [Histonet] Histonet Digest, Vol 151, Issue 1

2016-06-01 Thread Ingles Claire via Histonet
Not to mention that if you inhale too many fumes you will pass out. One of the original anesthetics. :) Claire From: Douglas Gregg via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, June 01, 2016 1:04 PM To: histonet@lists.utsouthwestern.edu

Re: [Histonet] PAS Stain

2016-05-05 Thread Ingles Claire via Histonet
I don't know, I believe Dr. Salk did the same with the polio vaccine. He even involved his family! Dedicated doctors... Claire From: Tony Henwood (SCHN) via Histonet [histonet@lists.utsouthwestern.edu] Sent: Thursday, May 05, 2016 6:33 PM To: Anne Murvosh

Re: [Histonet] PAS Stain

2016-05-05 Thread Ingles Claire via Histonet
The level of amylase in your saliva also depends on when you ate last. If you're spitting on slides after you just ate, the reaction will be weaker as the amylase will have been used up on lunch digestion. (also, you need to be careful about having your lunch salad contaminate the slide... :) Cl

Re: [Histonet] Running a histo lab without a histotech?

2015-10-23 Thread Ingles Claire via Histonet
Not to my knowledge. We have 3 derm/Mohs labs here in our system. There are only two of us who have our certifications. Heaven knows the world (or the lab for that matter) doesn't stop revolving when WE go on vacation. The third lab is a one person show and she isn't certified. Just so all the C

Re: [Histonet] are you desperate enough to hire a B.Sc. graduate?

2015-08-10 Thread Ingles Claire via Histonet
We have had this problem for years. We are more than willing to train someone. We are a small lab so a bigger concern is if they will fit in with the rest of us personality wise. Not that we just hire anyone willy-nilly, but many people with BS's have some lab experience/background anyway. We ju

Re: [Histonet] Manual Cassette Labelling

2015-08-06 Thread Ingles Claire via Histonet
We use Secureline Marker II/superfrost Histoprep pens from Fisher. Their item # is 14-905-30 for black, 14-905-35 for red. We have never had a problem with them smearing or fading on the cassettes no matter what we subject them to. Claire From: Joanne Cla

Re: [Histonet] Marciafava-Bignami Disease

2015-08-04 Thread Ingles Claire via Histonet
I know I watch my exposure to Formalin and Xylene especially. I was diagnosed with NASH in 2007 (who knows how long before then, since I am asymptomatic) when I had a Pituitary Adenoma that had hemorraged (Boy talk about a headache!) Anyway, I have always wondered about the cause. I thought it w