[Histonet] Staining paraffin blocks of evans blue treated mice with Sirius red

Hi Histonets. Can i stain slides from praffin blocks of Evans blue treated mice with Sirius red ? Does the Sirius red might be effected from the Evans blue treatment? Have someone done it before, or have a reference for it ? Thank you all, Itai ___ His

[Histonet] Paraffin slides storage

Hello Histonets, I have some questions about paraffin slides storage. 1) For how long do you store paraffin slides after sectioning ? 2) At what conditions do you store the slides ? Itai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http:/

[Histonet] How to store paraffin blocks ?

o the paraffin blocks and the tissue ? After sectioning, How do you store the slides, and for how long ? Thank's Itai Moshe ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Leaving sample over night in ETOH 70% during fixation

> fixation time is carefully controlled…I do not think that the methanol in > the formalin could affect anything adversely, also 10% neutral buffered > formalin is the fixative of choice for most labs, I have never had > a problem with it. > > ** ** > >

Re: [Histonet] Leaving sample over night in ETOH 70% during fixation

ng tissue overnight in 70% ethanol at 4C should have no detrimental > effect n the tissues, why 4C overnight with Bouin's?? > > -Original Message- > From: histonet-boun...@lists.utsouthwestern.edu [mailto: > histonet-boun...@lists.utsouthwestern.ed

[Histonet] Leaving sample over night in ETOH 70% during fixation

Dear All, Does leaving the samples (diaphragm, liver) over night in ethanol 70% at 4C during the fixation process, will be better for the tissue fixation, and does not harm the sample ? Does the fixation process should be done straight forward from the first step to the last one without any over ni

[Histonet] Re: Picro sirius red, fast green connective tissue stain

igin=gateway&_sort=d&_docanchor=&view=c&_acct=C32999&_version=1&_urlVersion=0&_userid=626711&md5=805ac6cd7adeb0d8284a07f0be44dcb8&searchtype=a> Itai 2011/3/8 John Kiernan > Dear Itai Moshe, > > Can you explain the staining method you described in some Histonet > correspo

Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining

t; * * *2010/9/18 Jack Ratliff *** > *Dorn and Hart Microedge is now selling a Weigert's Hematoxylin kit. Maybe > you could try it out and see if your results improve. > > **www.dornandhart.com* <http://www.dornandhart.com/>* > ** > Jack* * * *On Sat, September 18, 2010 9:03 am, I

Re: [Histonet] Re: Masson's trichrom - problem with nuclei staining

minutes. > > > > Best Regards, > > Patty Lott > > 2010/9/16 Jack Ratliff > >> The tap water rinse is in reference to running tap water (not directly on >> the slide sections) while the slides sit in a make-shift water bath. This is >> done over a

[Histonet] Masson's trichrom - problem with nuclei staining

Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 With sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT107

[Histonet] Re: Problem with liver fixation

Hi Histonet's I'm using Masson's trichrome to stain paraffin mice diaphragm fixed with PFA. I'm using this protocol: http://www.bcm.edu/mcb/rosenlab/index.cfm?pmid=12997 with sigma masson's kit (#HT15) and Weigert's Iron Hematoxylin Set (#HT1079) The staining is beautiful, but i can't see the nucle

[Histonet] Re: Problem with liver fixation

h dehydration and clearing step, > and 2 paraffin steps for one hour each with mouse tissue. Good luck! > > Andrea Marion > > amario3 &at& uic &dot& edu > Graduate Student > University of Illinois at Chicago > > > > Rene J Buesa histonet@list

[Histonet] Problem with liver fixation

Dear All, I'm trying to use liver sections in paraffin blocks for IHC, but get no signal at all, doesn't matter what antibody i'm using. I'm using section about 3mm thick (the natural thickness of mouse liver) and about 5-7mm Width and Length. My fixation protocol is like this: 1) immediately aft

[Histonet] picro sirius red, sirius red connective tissue stain[Scanned]

DDW and empty). 7) Check in microscope, if the colors are not strong enough, repeat from step 2 to 7. That's it. -- Itai Moshe Mark Pines lab Institute of Animal Sciences,Volcani Center. Dept. of Animal Sciences, School of Veterinary Medicine, The Hebrew University of Jerusalem I

[Histonet] Aniline blue

What type of collagen aniline blue is staining ? Thanks in advance Itai ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet