Re: [Histonet] Antibody diluent

2021-03-25 Thread Jeanine Ronkowski via Histonet
Scott:I’m pretty certain that you wouldn’t need to completely re-validate after changing diluents, but you may have to change the dilution factor slightly.   It’s my understanding that a change like this only requires staining a few known positive samples, verifying that the results you obtain ar

Re: [Histonet] Antibody diluent

2021-03-23 Thread Jeanine Ronkowski via Histonet
All:Our lab uses a ton of concentrated antibodies and we’ve found that there’s really no such thing as a universal diluent like the ones offered by Leica and Agilent.  We get ours from Biocare, they offer five different diluents with different pHs and chemical makeups and we use three of these.

[Histonet] QIHC Exam Preparers

2020-09-24 Thread Jeanine Ronkowski via Histonet
Hi Histo-peeps:I’m just wondering if someone can shed some light on who prepares the QIHC exam.  Everyone I’ve talked to about the test says that all of the questions are tricky and cover a lot older topics rather than more relavent information.  So how can we get some folks who understand today

Re: [Histonet] Questions on how to operate a ThermoFisher Lab Vision autostainer 3602D

2019-10-25 Thread Jeanine Ronkowski via Histonet
Katherine:Our lab did the same thing a few years ago and I received operating instructions for this system from a sales rep with Biocare that I know.  I'll send this document to you offline.  I can also put you in touch with the guy from Biocare.  As Colleen said, Biocare doesn't sell or support

[Histonet] Histonet - IHC Manual staining

2019-01-18 Thread Jeanine Ronkowski via Histonet
Hi Dave:Our lab has used reagents from Biocare Medical for a very long time. They’re packaged in 6ml and 25 ml vials and ideal for hand staining. And their technical support is great tooJeanine Hello Histoneters - Does anyone still do manual IHC's? If so, which IHC staining kits would you reco

[Histonet] IHC System from StatLab

2018-06-14 Thread Jeanine Ronkowski via Histonet
I’m pleased to report that I was finally contacted by a representative from StatLab that provided me with the information I was seeking.  I realize that their system may not be fully released, but I didn’t think it would be that difficult for them to provide information.  Turns out that this ins

Re: [Histonet] IHC System from StatLab

2018-06-12 Thread Jeanine Ronkowski via Histonet
All:For those of you who saw my posting last week and we’re wondering about the outcome, I’m sorry to say that I’ve received very little useful feedback.  Two individuals from StatLab sent me separate, cryptic messages indicating that they would be be happy to either meet with me or provide a de

[Histonet] IHC System from StatLab

2018-06-07 Thread Jeanine Ronkowski via Histonet
I’ve heard from a number of people that Stat Lab plans to release a new instrument for immunostaining and I was wondering if someone, including the folks at StatLab, might be able to provide more information.Thx,Jeanine Sent from Yahoo Mail for iPhone ___