[Histonet] Cryostat sectioning of frozen leaves

2016-08-09 Thread Jo-Ann Bader, Ms. via Histonet
I am trying to cut frozen leaf sections at 6 and 8 microns. All I am getting is bunched up leaf. What is the trick/ I am cutting at -20 should be cutting at a lower temp and cutting thicker? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm

Re: [Histonet] Histonet Digest, Vol 151, Issue 18

2016-06-21 Thread Jo-Ann Bader, Ms. via Histonet
We are happy the ABCAM CD3 Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270 From: histonet-requ...@lists.utsouthwestern.edu

[Histonet] ORO tissue falling off

2015-04-15 Thread Jo-Ann Bader, Ms.
We are having difficulty with a particulate set of very, very fatty mouse livers. The normal livers from this set stay on the slides the fatty livers fall off. We have used different types of charged slides and we have even tried to drench the charged slides in Stay-On, dry them and then put

[Histonet] Whole mouse fixation

2014-05-05 Thread Jo-Ann Bader, Ms.
Good afternoon Histonetters, I have a client who wants us to process,embed and cut saggital sections of a whole mouse which is 8-9 months old. I have a pretty good idea of what needs to be done but I would like some feedback from those who have more experience than I do. Fixation time, and

[Histonet] Micro Tissue Arrayer

2013-07-23 Thread Jo-Ann Bader, Ms.
We are currently in the market for a Micro Tissue Arrayer. I would like to hear from other labs that have one, which one do you have and is it easy to getr service for them. Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal,

[Histonet] Frozen bone sections

2013-05-13 Thread Jo-Ann Bader, Ms.
I have been asked to cut frozen mouse tibia. I have never cut frozen bone I could use some advice. Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave. W - Rm 312 Montreal, QC, Canada H3G 1Y6 Tel: 514-398-8270

[Histonet] Fibrin staining

2013-05-10 Thread Jo-Ann Bader, Ms.
We have a client who is interested in studying both fibrin loose in the mouse tissue as well as intravascular (arterial or venous). It has been suggested I do either a Picro-Mallory or a Martius, Scarlet Blue (MSB) stain. Is there any other stains I could do, which is the most popular?

[Histonet] CD31 problewms

2013-02-14 Thread Jo-Ann Bader, Ms.
We are having problems getting our CD31 to work well on our NBF fixed mouse tissue. There is staining but it is very pale. The CD31 comes from Bio-care and we are are using the IntelliPATH stainer. We have tried: - Trypsine 37C 15 min / Trypsine RT 30 min - Trypsine (1:2 and 1:3) - With

[Histonet] Slide Labelers

2012-09-10 Thread Jo-Ann Bader, Ms.
Good Morning All, We are in the market for a new slide labeler. We are not happy with our current one. I would like to hear the oppinion from other labs on the slide labelers they are using. Thank you Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1160 Pine Ave.

[Histonet] Stripped HE's - HELP!

2011-07-07 Thread Jo-Ann Bader, Ms.
Hi All, Over the past year we have had a recurring problems with our HE's. The come out of our Gemini Stainer stripped but not on every run; we can go for months without a problem and then all of a sudden it is back again. The stripping is in the Eosin, our pathologist confirms the the

[Histonet] Heck

2011-03-22 Thread Jo-Ann Bader, Ms.
Hi Helen, Can you check the blocks I sent you and see if you have 3 blocks marked EXP 0012. We have so many back up systems in place that it is almost as if I never received these blocks but I have to check anyway. Thanks Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill

[Histonet] Short term tissue storage PBS???

2011-02-23 Thread Jo-Ann Bader, Ms.
Hi All, It has come to my attention recently that some of our histology clients are submitting their samples in PBS after fixation, instead of 70% alcohol. When I ask them why they don't know and say they have been told to. I have always followed the procedure that after fixation specimens

[Histonet] Mouse Eyes

2009-07-29 Thread Jo-Ann Bader, Ms.
We have a new project pending with mouse eyes. The lens must remain intact. What is the best method to process, embed and cut them? Paraffin or glycol methacrylate? Jo-Ann Bader Histology Co-Ordinator Goodman Cancer Centre McGill University 1600 Pine Ave W. Room 312 Montreal, QC, Canada

[Histonet] SOPS

2009-06-09 Thread Jo-Ann Bader, Ms.
Good Day All, We have currently merged two histology groups to form a Histology Core. We are currently going over all our SOP's and we were wondering if some of you would share your SOP'S so we could have a better idea if we are in the same ball park as others. We are looking mainly at

[Histonet] Acidified Hematoxylin

2008-10-20 Thread Jo-Ann Bader, Ms.
Good Morning Everyone, We are in dire straights We are coming to the end of out Acidified Harris Hematoxylin and I was just informed that the company that produces it will not start production until November. We will never make it. Does anyone know where I can get some? Jo-Ann Bader

RE: [Histonet] Baker's Fluid

2008-10-20 Thread Jo-Ann Bader, Ms.
PROTECTED] Sent: Mon 10/20/2008 11:16 AM To: Jo-Ann Bader, Ms.; Histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Baker's Fluid This was in the archives. Hope it helps! Joyce Weems Pathology Manager Saint Joseph's Hospital 5665 Peachtree Dunwoody Rd NE Atlanta, GA 30342 Please note new phone