[Histonet] i thought the joke was funny

Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pa

[Histonet] Fite for Nocardio

I did this stain at AFIP with great success. Still use peanut oil and xylene, carbol fuchsin but instead of using acid alcohol with HCL, using sulfuric acid. Works great. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street

[Histonet] commercial antibody diluent with carrier proteins SUGGESTIONS?

Hi all, I would like to get a commercially prepared antibody diluent with carrier proteins. I have been told Dako has a decent one but I am trying to find a different vendor. I see a few places with diluent but none that have background reducing substances/carrier protein type thing. Nick M

[Histonet] Brown and Brenn

You can switch to Tartrazine. Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc

[Histonet] PAS Rat Testes

If the path does not want to use Davidsons and still wants the acetic acid, perhaps Carnoys, Bouins, Methacarn? Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGI

[Histonet] Repair techs for very old Shandon Tissue Processor, (Roosevelt was in office)

I have an old Shandon tissue processor that is so old, they just called in tissue processor, I think Kennedy was in office, and the serial number is -10. THe machine has been donated and passed down and I am having a hard time getting to work. I t worked once, but now it just keeps giving me ev

[Histonet] conservation histotech

Well you can always run your lab in the most green way possible using recyling and reuse management of all materials in the lab. In Md there are a few state labs with coveted positions that only open up through the death or retirement of a tech because they are so rewarding. The closest thing I

[Histonet] disposable blade system for microm 505e or

Hi netters, I have a system that uses full sized microtome blades for the Microm 505E cryostat. I think it would be cheaper to order the item I have seen before that us old timers used when disposable blade systems first came on the seen. The blade holder looks and feels like an old time microto

[Histonet] good source for APES to make silane treated slides?

Nick Madary, HT/HTL(ASCP)QIHC George Washington University Pathology Core Laboratory Ross Hall, Room 706 23rd and I Street NW Washington D.C. 20037 202.994.8916 pat...@gwumc.edu BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Joseph Madary EMAIL;WORK;PREF;NGW:pat...@gwumc.edu N:Madary;Joseph ORG:;Pa

[Histonet] oct to paraffin

If you can spare some nbf the best thing to do would be to place the oct block right into NBF and use that as the "wash" and then move onto a fresh change of NBF. I would avoid straight water. NBF has enough water in it to rinse off the NBF, 1 or 2 changee for 15 minutes each is more than enough

[Histonet] interview questions

I think it is great that you are using the histonet this way to ask probing questions. SInce they are replacing you, I would get a feel for how they would do your job by telling them the general taskings and letting them tell you what they know about all of the taskings. I have also found that s

[Histonet] recycling formalin

There are lots of systems out there now. There are filtertion systems that require very little on the part of the tech beyond changing the filter. There are other systems a bit more complicated that require adjusting the strength, and rebuffering(not that hard). In all cases the systems pay for the