We are trouble-shooting cryosectioning mouse lung tissue. Often the lung
section tears or breaks apart during sectioning. In the past, if the lung
section is proving difficult to section, we take the OCT-embedded tissue and
re-embed it back into OCT (basically put fresh OCT into the original
Hi,
We are performing some immunofluorescence staining on mouse lung tissue. We
are getting some nice positive staining with some of our initial antibodies
(procollagen, cytokeratin).
We would like to minimize the amount of background staining we are getting.
We are titering our primary
We are currently starting up some IHC on frozen tissue sections. After
staining with different fluorescent antibodies, we end with applying DAPI
w/Prolong gold and then coverslipping. We would like to seal the coverslip so
that we can keep the slides longer.
Any suggestions on where and how
